This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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Kit Specifications
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Maximum Binding Capacity Per Well
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50 μg
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Maximum Loading Volume Per Well
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500 μL
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| Size of RNA Purified |
All sizes, including small RNA < 200 nt
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| Time to Complete 96 Purifications |
30 minutes
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| Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster) |
15 μg
50 μg 9 μg 26 μg 1 μg |
| Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue |
100 µL
150 µL
1 x 109 cells
1 x 108 cells
40 mg 40 mg |
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Comparison of Plate Dimensions
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| Total RNA 96-Well Plates | Length (cm) | Width (cm) | Height (cm) |
| Cat# 24300 / Cat# 24370: 96-well plate | 12.8 | 8.6 | 3.4 |
| Cat# 24300 / Cat# 24370: 96-well plate assembled with collection plate | 12.8 | 8.6 | 4.4 |
| Cat# 24350/ Cat# 24380: 96-well plate (deep well format) | 12.8 | 8.5 | 3.8 |
| Cat# 24350/ Cat# 24380: 96-well plate (deep well format) assembled with collection plate | 12.8 | 8.6 | 5.7 |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.