RNA/Protein Purification Plus Kit – 48200: Rapid, Sequential Macromolecule Isolation
Simultaneous profiling of the transcriptome and proteome often poses a significant challenge, typically requiring researchers to split valuable samples or rely on hazardous, phenol-based extraction methods that can compromise yield. The RNA/Protein Purification Plus Kit (48200) offers an innovative, rapid spin-column solution for the sequential isolation of both total RNA (including miRNA) and total proteins from the exact same sample. This advanced system eliminates the need for sample fractionation, ensuring that your downstream analytical data—whether derived from cultured cells, tissues, blood, or plant material—is directly comparable and highly reliable.
Operating entirely without the use of toxic phenol or chloroform, the kit employs a proprietary ion-exchange resin to selectively bind nucleic acids whilst allowing proteins to flow through. Following a swift and non-enzymatic genomic DNA removal step using the dedicated gDNA elimination column, high-purity RNA is eluted. The initial protein-rich flowthrough is then easily pH-adjusted and loaded back onto the column, permitting the secure capture, washing, and subsequent elution of functional proteins. By completing the entire sequential purification in under 30 minutes, this kit dramatically accelerates workflows and preserves the absolute integrity of your macromolecules.
Key Benefits:
- Sequential Purification: No sample splitting required. Isolate both RNA and proteins from a single unified lysate, providing absolute confidence when correlating gene expression with protein translation.
- Exceptional Efficiency: Complete in under 30 minutes. The streamlined protocol bypasses tedious protein precipitation, pellet resuspension, and organic solvent extractions, saving significant laboratory time.
- High-Fidelity Outputs: Ready for immediate analysis. Purified RNA is highly suited for NGS, microarrays, and RT-qPCR, whilst proteins are eluted in a highly compatible buffer ready for Mass Spectrometry, Western blotting, and ELISA.
- Built-In gDNA Removal: Enzyme-free DNA elimination. Includes specialised gDNA removal columns that effectively strip out contaminating genomic DNA prior to RNA isolation, ensuring pristine transcripts.
- Optimised for Precious Samples: Maximise limited inputs. Perfectly designed for processing challenging, small-volume samples such as biopsy materials or single-cell foci where conserving material is paramount.
Technical Insights
Spin-Column Chromatography vs. Traditional Methods
Traditional multi-omics isolation techniques often depend on aggressive solvent separation (such as TRIzol) which inherently splits the sample into distinct phases, risking cross-contamination, selective loss of small RNAs, or protein denaturation. In contrast, this kit utilises a highly specific matrix that binds macromolecules based on ionic concentration. By capturing RNA first and cleanly sequestering the protein fraction in the flowthrough for subsequent capture, the system maintains a seamless, non-destructive workflow. This precise control over binding conditions ensures exceptional recovery rates without exposing the sample to harsh, function-altering chemicals.
Specifications
- Product Type: Spin-Column RNA and Protein Isolation Kit
- Maximum Column Binding Capacity: 50 µg for RNA, 200 µg for Protein
- Maximum Column Loading Volume: 650 µL
- Processing Time: Less than 30 minutes for 10 purifications
- RNA Size Purified: All sizes, including small RNA and miRNA (< 200 nt)
- Sample Compatibility: Cultured animal cells, tissues, blood, bacteria, yeast, fungi, and plants
- Kit Format: 50 Preparations
- Storage: Room temperature (Protein Loading Dye requires -20°C storage after DTT addition)