RNA/DNA/Protein Purification Plus Micro Kit: Maximising Multi-Omic Data from Precious Samples
The RNA/DNA/Protein Purification Plus Micro Kit is an advanced solution designed for the sequential isolation of total RNA, genomic DNA, and total proteins from a single biological sample. In many research scenarios, particularly when working with microdissected tissues (LCM), stem cells, or circulating tumour cells (CTCs), sample material is extremely limited. This kit addresses the critical challenge of extracting multiple macromolecules without the need to split or fractionate the initial sample, ensuring that researchers can perform comprehensive genomic, transcriptomic, and proteomic analyses from the exact same cellular source.
Utilising a rapid spin-column chromatography method based on a proprietary resin, the kit facilitates the sequential binding and elution of nucleic acids and proteins. The process begins with sample lysis, followed by the sequential capture of DNA and then RNA on specialised micro-columns. Finally, the proteins are purified using the same workflow. This technology eliminates the risk of inconsistent results that can occur when comparing data from separate sample aliquots, providing a more reliable foundation for systems biology and integrated molecular studies.
Key Benefits:
- Integrated Workflow: Sequential isolation from one sample. Eliminates the need to divide precious samples, ensuring 100% of the material is used for RNA, DNA, and protein extraction.
- Optimised for Micro-Samples: Ultra-low elution volumes. The specialised micro-columns allow for elution in as little as 20 µl for nucleic acids and 50 µl for proteins, providing high concentrations for sensitive downstream assays.
- Rapid Processing: Complete purification in under 30 minutes. The streamlined spin-column protocol significantly reduces bench time compared to traditional phenol-chloroform or multi-kit methods.
- High Integrity Yields: Consistent, high-quality results. Purified macromolecules are of the highest integrity, comparable to those isolated using dedicated, single-analyte kits.
- Downstream Ready Proteins: Buffer-based elution. Proteins are eluted directly into a specialised buffer, eliminating the need for pellet resuspension and making them immediately suitable for Western blotting, mass spectrometry, and quantification.
Technical Insights
The RNA/DNA/Protein Purification Plus Micro Kit is particularly advantageous for researchers involved in systems biology where the correlation between the genome, transcriptome, and proteome is essential. By deriving all three components from a single foci of cell culture or a small biopsy, experimental error associated with sample heterogeneity is virtually eliminated.
Specifications
- Catalogue Number: P4-0174
- Sample Types: Cultured animal cells, small tissue samples, LCM, stem cells, sorted cells, and CTCs
- Processing Time: Less than 30 minutes
- Minimum Elution Volume (RNA/DNA): 20 µl
- Minimum Elution Volume (Protein): 50 µl
- Downstream Applications: qPCR, NGS, Microarrays, Southern/Northern Blotting, Western Blotting, Mass Spectrometry
- Storage Conditions: All solutions should be kept tightly sealed and stored at room temperature