RNA/DNA/Protein Purification Plus Kit: Unified Multi-Omic Extraction
The RNA/DNA/Protein Purification Plus Kit provides an efficient and rapid spin-column method for the sequential isolation of total RNA, genomic DNA, and total proteins from a single biological sample. Designed to handle a diverse range of starting materials—including cultured animal cells, tissues, blood, bacteria, yeast, fungi, and plants—this kit is an essential tool for researchers performing multi-omic studies. By extracting all three macromolecules from the same sample, it eliminates the inherent variability associated with sample splitting, ensuring more accurate correlations between the genome, transcriptome, and proteome.
The purification process utilises a dual-column system to ensure maximum purity and yield for each analyte. Genomic DNA is first captured on a specific DNA-binding column, followed by the isolation of total RNA using a second column featuring advanced silicon carbide technology. This resin is particularly effective at binding all RNA species, including small RNAs and miRNAs, without the need for phenol or chloroform. Finally, proteins are captured and eluted from the same RNA column. This streamlined workflow allows for the complete preparation of all three components in a fraction of the time required by traditional methods.
Key Benefits:
- Comprehensive Sequential Isolation: Three analytes, one sample. Allows for the simultaneous study of DNA, RNA, and protein from a single source, ideal for precious or limited samples.
- Superior RNA Recovery: Silicon carbide technology. Provides high-affinity binding for all RNA sizes, including microRNA (miRNA), without the use of hazardous chemicals like phenol.
- Broad Sample Compatibility: Versatile application. Optimised for a wide array of inputs, from animal tissues and blood to plant matter, bacteria, and fungi.
- Ready-to-Use Proteins: Direct elution in buffer. Proteins are eluted in a stable buffer, making them immediately ready for quantification, Western blotting, ELISA, or mass spectrometry without further clean-up.
- Fast and Reliable: Streamlined spin-column protocol. Reduces the complexity of multi-omic workflows, providing high-integrity yields in a rapid, repeatable format.
Technical Insights
The use of silicon carbide resin in the RNA/DNA/Protein Purification Plus Kit represents a significant advancement over traditional silica-based methods. This technology ensures that even the smallest RNA molecules are retained, providing a more representative snapshot of the transcriptome. Because the proteins are purified following RNA elution, they remain in a native or near-native state, facilitating accurate downstream proteomic analysis.
Specifications
- Catalogue Number: P4-0140
- Sample Types: Animal cells/tissues, blood, bacteria, yeast, fungi, plants
- Technology: Dual-column spin chromatography (Silicon Carbide)
- Extraction Type: Sequential (DNA then RNA then Protein)
- Downstream Applications: NGS, qPCR, Southern/Northern Blotting, Western Blotting, ELISA, Mass Spectrometry
- Storage Conditions: Store at room temperature (15-25°C)