FastGene® 2x IC Green Universal qPCR Mix: Universal Precision for Real-Time PCR
The FastGene® 2x IC Green Universal qPCR Mix with Separate ROX is a high-performance master mix designed to simplify real-time PCR workflows while delivering exceptional sensitivity. By providing the ROX reference dye in a separate tube, this kit offers true universal compatibility, addressing the challenge of stocking multiple specific mixes for different thermal cyclers. Whether you are analysing gene expression or quantifying genomic DNA, this mix ensures reliable quantification across a broad dynamic range.
At the core of this formulation is the proprietary IC Green intercalating dye, which offers significant advantages over traditional SYBR® Green I. IC Green binds specifically to double-stranded DNA with a high extinction coefficient, generating a robust fluorescent signal while inhibiting PCR significantly less than legacy dyes. Combined with a highly purified Taq DNA polymerase and an optimised buffer system, the mix supports rapid extension rates and precise amplification, even with low-copy number templates.
Key Benefits:
- Universal Compatibility: One mix for all platforms. The kit includes a separate tube of ROX reference dye (50 μM), allowing users to adjust the concentration to match High ROX, Low ROX, or No ROX requirements of any qPCR instrument.
- Superior Sensitivity: High signal-to-noise ratio. The IC Green dye is engineered for intense fluorescence and minimal inhibition, allowing for the detection of genes with very low expression levels.
- Optimised Buffer Chemistry: Robust amplification. The specialised buffer formulation stabilises the reaction, ensuring high efficiency and reproducibility across varied template concentrations.
- Rapid Protocols: Faster time-to-result. The high-speed polymerase activity enables shortened run times without compromising the specificity or accuracy of the melt curve analysis.
Technical Insights
Unrivalled Signal Strength
The FastGene® IC Green dye distinguishes itself through superior chemical stability and non-inhibitory binding. Unlike traditional intercalating dyes that can stall polymerase activity at high concentrations, IC Green allows for efficient chain extension. This results in earlier Cq values and sharper melt peaks, facilitating precise discrimination between specific amplicons and primer-dimers.
Universal Instrument Application
By keeping the ROX additive separate, this mix eliminates the need to purchase platform-specific reagents. Users simply add the required amount of ROX to the master mix prior to the run—typically 1 μL for Low ROX instruments (e.g., ABI® 7500) or High ROX instruments (e.g., ABI® StepOne™), ensuring seamless integration into existing laboratory workflows.
Specifications
- Concentration: 2x Master Mix
- Detection Method: Intercalating Dye (IC Green)
- Reference Dye: Separate ROX (50 μM) provided
- Polymerase: FastGene® Taq DNA Polymerase
- Storage Conditions: -20 °C (avoid repeated freeze-thaw cycles; protect from light)
- Shelf Life: Stable for up to 12 months when stored properly
- Applications: Gene expression analysis, SNP genotyping, Copy Number Variation (CNV)