P4-0058
Total RNA Purification Kit – 17200

For rapid purification of total RNA - including microRNA, without phenol. Very sensitive & linear down to a few cells without the need for carrier RNA
P4-0058-Image
Catalogue NumberDescriptionPack SizePrice
P4-0058Total RNA Purification Kit – 1720050 Preps£287.00
This kit is suitable for the isolation of Total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Specification

Specification
Maximum Binding Capacity Per Column Up to 50 μg RNA Maximum Loading Volume Per Spin Column 650 μL Size of RNA Purified All sizes, including small RNA < 200 nt Time to Complete 10 Purifications 20 minutes Maximum Amount of Starting Material Liver Tissue, 10 mg Heart Tissue, 5 mg Kidney Tissue, 10 mg Brain Tissue, 25 mg Lung Tissue, 10 mg Spleen Tissue, 20 mg Whole Blood, 100 µL Plasma/Serum, 200 µL Hela Cells, 3 x 106 CHO , 3 x 106 Yeast, 1 x 108 cells E.coli, 1 x 109 cells Bacteria, 1 x 109 cells Fungi, ≤ 50 mg Plant Tissues, ≤ 50 mg Plant Cells, 5 x 106 cells Viruses, ≤100 µL of viral suspension Average Yield HeLa Cells (1 x 106 cells) E. coli (1 x 109 cells) 15 μg 50 μg *For isolating Total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (P4-0061-25700) Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Documentation

Literature
P4-0058 Literature 17200
Protocol
P4-0058 Protocol 17200
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