Total RNA Purification Kit: Comprehensive RNA & miRNA Isolation
The Total RNA Purification Kit provides a rapid and robust solution for the isolation of high-quality total RNA from a diverse range of biological samples. Whether working with cultured cells, bacteria, yeast, viruses, or complex bodily fluids such as plasma, serum, and CSF, this kit ensures the recovery of superior-grade RNA. By eliminating the requirement for hazardous phenol-chloroform extractions or time-consuming alcohol precipitations, it streamlines laboratory workflows while maintaining the highest standards of sample integrity and user safety.
Central to the kit’s performance is its proprietary resin-based spin column technology. Unlike traditional silica-based methods that often show bias against small RNA species, this system captures all RNA sequences—from large mRNA and lncRNA down to microRNA (miRNA) and siRNA—at an equal rate. The binding mechanism is independent of GC content, ensuring a truly representative RNA profile that is essential for accurate downstream quantification in NGS, qRT-PCR, and microarray analysis.
Key Benefits:
- Full Transcriptome Coverage: Capture all RNA sizes in a single fraction. Successfully isolates large mRNA (>200 nt) and small RNA (<200 nt) simultaneously, including miRNA and lncRNA.
- Phenol-Free Protocol: Enhanced safety and purity. No hazardous chemicals are required, preventing phenol carryover which can inhibit sensitive enzymatic reactions.
- Unmatched Sensitivity: Optimised for low-input samples. The kit is linear and sensitive down to just a few cells, removing the need for carrier RNA in most applications.
- Versatile Sample Compatibility: One kit for all sample types. Validated for use with animal cells, tissues, bacteria, yeast, and difficult bodily fluids like saliva and blood.
- High Integrity RNA: Excellent RIN values. Produces purified RNA with high A260/A280 ratios, ready for the most demanding molecular biology applications.
Technical Insights
The Total RNA Purification Kit is engineered to overcome the limitations of standard RNA extraction. While many kits lose small RNA fragments during the washing steps, the specialised resin used in these columns maintains a high affinity for small sequences regardless of their secondary structure or nucleotide composition. This ensures that the resulting RNA library is an exact reflection of the original biological state.
Specifications
- Format: Spin Column
- RNA Size Range: Full range (including miRNA >10 nt)
- Processing Time: ~20 minutes
- Shelf Life: 2 years at room temperature
- Downstream Applications: NGS, qRT-PCR, RT-PCR, Microarrays, Northern Blotting
| Catalogue No. | Product | Contents/Specifications |
|---|---|---|
| P4-0058 | Total RNA Purification Kit (50 Preps) | Standard pack size for routine lab isolation; includes all columns and buffers. |
| P4-0069 | Total RNA Purification Kit (100 Preps) | Medium throughput pack for consistent project workflows. |
| P4-0227 | Total RNA Purification Kit (250 Preps) | Bulk size for high-frequency testing and core facilities. |
| P4-0426 | Total RNA Purification Kit (500 Preps) | Economy bulk pack for high-throughput screening and large-scale studies. |