EZCollect Saliva RNA Collection Device – RU69800

Norgen’s EZCollect (Easy Collect) Saliva RNA Device provides a safe and rapid method for home-based collection, and preservation of saliva RNA.

1. Simple and non-invasive home-based saliva collection
2. Inactivation of pathogens and viruses, including SARS-CoV-2
3. Preservation of RNA in saliva samples at ambient temperature. Intended for Research Use Only

Collection Protocol

1. 

   Deposit saliva into the Collection Tube up to the indicated line.

   If you notice excess bubbles, eliminate them by gently tapping the tube on a hard surface.

2. 

   Remove the Collection Funnel. Cap the Collection Tube by pushing down and twisting until secured, and the Blue Saliva RNA Preservative will be released.

   Discard or recycle the Collection Funnel.

3. 

   Shake well for 10 seconds to mix the saliva with the Blue Saliva RNA Preservative

   The saliva sample is preserved and pathogens & viruses, including SARS-CoV-2, are now inactivated.

4. 

   The Collection Tube, containing the saliva sample is ready for transport.

   

   Figure 1. Stability of RNA Preserved in Norgen’s Saliva RNA Preservative at room temperature for up to 2 months.Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen’s Saliva RNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 2 months. Saliva RNA was subsequently isolated at day 0, day 4, day 6, day 16, 1 month and 2 months from 0.25 mL of the saliva/preservative sample using  supplementary protocol associated with Norgen’s Total RNA Purification Kit (Cat. 17200). For visual analysis, 7.5 µL from 50 µL elution was mixed with RNA loading dye and was run on a 1x MOPS agarose gel. RNA integrity was maintained after the saliva samples were stored up to 2 months at ambient temperatures in Norgen’s Saliva RNA Preservative.

   

   Figure 2. Stability of three house keeping genes over a 2 month period.GAPDH, Human 18S rRNA and beta actin genes were monitored from saliva samples stored at ambient temperature with Norgen’s Saliva RNA Preservative. RNA was isolated from 250 µL of preserved samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgen’s Total RNA Purification Kit (Cat. 17200). Next, 4 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 Touch^TMReal-Time PCR Detection System (Bio-Rad). All of the genes were preserved and stable over 2 month period.

   Storage Conditions and Product Stability
   This device should be stored at room temperature for up to 2 years without any reduction in performance prior to Saliva Sample Collection. After the Saliva Sample Collection, the saliva RNA in preserved samples is stable for up to 2 months at room temperature (15-30°C).

   Component	Cat. RU69800 (1 Unit)
   Saliva Collection Funnel	1
   Collection Tube Containing Norgen’s Saliva RNA Preservative	1
   Collection Tube Cap	1