Total RNA Purification Plus Kit: High-Purity RNA Recovery with Integrated gDNA Removal
The Total RNA Purification Plus Kit is a high-performance solution designed for the rapid extraction of high-quality total RNA, including small RNA species like microRNA (miRNA). This kit addresses the critical challenge of genomic DNA (gDNA) contamination, which can compromise downstream molecular assays. By incorporating a dedicated gDNA removal column, the system provides a rapid and efficient alternative to enzymatic DNase digestion, ensuring the integrity of the sample while significantly streamlining the laboratory workflow.
Utilising Norgen’s proprietary resin technology, the kit operates through a spin column chromatography method that does not require phenol or chloroform. This advanced mechanism ensures that all RNA molecules—regardless of their size, sequence, or GC content—are bound and eluted with equal efficiency. This bias-free recovery is essential for quantitative applications, as it provides a true representation of the cellular transcriptome, from large mRNA and lincRNA down to 10 nt small RNAs.
Key Benefits:
- Integrated gDNA Removal: Rapid genomic DNA elimination. The inclusion of a gDNA removal column eliminates the need for time-consuming and costly enzymatic DNase treatments.
- Bias-Free Small RNA Recovery: Comprehensive transcriptomic profile. Recovers all RNA species, including microRNA, without bias towards size or GC content, ensuring accurate representation.
- Phenol-Free Technology: Safer laboratory environment. The column-based workflow eliminates the need for hazardous organic solvents like phenol and chloroform.
- Broad Sample Compatibility: Versatile application range. Efficiently process cells, bacteria, yeast, viruses, and various biofluids including plasma, serum, blood, and saliva.
- Superior RNA Integrity: Optimised for sensitive downstream assays. Produces high-quality RNA with excellent RIN values, suitable for NGS, microarrays, and qRT-PCR.
Technical Insights
Optimised Workflow for Downstream Precision
The Total RNA Purification Plus Kit simplifies the sample preparation pipeline by combining lysis, gDNA removal, and RNA binding into a seamless, 20-minute procedure. The gDNA removal column is specifically engineered to trap large genomic DNA fragments while allowing the total RNA to pass through into the second spin column. This dual-column approach results in higher RNA purity and more consistent performance in quantitative RT-PCR, where residual gDNA can otherwise lead to false-positive signals or overestimation of transcript levels.
Specifications
- Catalogue No.: P4-0015 (50 Preps), P4-0016 (100 Preps)
- Binding Capacity: 50 µg
- RNA Size Range: All sizes, from 10 nt upwards
- Processing Time: 20 minutes
- Format: Spin Column
- Storage: Room Temperature (15-25°C)
- Shelf Life: 12 months
- Certification: For research use only