P8-0054
FastGene Scriptase II cDNA Synthesis Kit

Engineered reverse transcriptase with a mutation in the RNase H domain and higher thermal stability which is ideal for RT-qPCR and NGS. Supplied with Oligo dTs, random hexamers and RNase inhibitor
P8-0054-Image
Catalogue NumberDescriptionPack SizePrice
P8-0054FastGene Scriptase II cDNA Synthesis Kit100 Reactions£369.00
Engineered reverse transcriptases allowed the synthesis of cDNA from very low amounts of RNA. Mutations are inserted into the RNase H domain of the MuLV's reverse transcriptase. Therefore, by not degrading the RNA during the first-strand synthesis, a higher yield of full-length cDNA is obtained. Additionally, a higher thermal stability increases the robustness of the enzyme. The FastGene® Scriptase II is exactly one of those engineered enzymes. With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS.

Specification

Specification
Quantification of Gene Expression. RT-qPCR. Next Generation Sequencing. Low RNA concentration. Difficult templates. The FastGene® Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12 kBp. The FastGene® Scriptase II delivers superior cDNA templates for downstream applications, e.g. qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.
Figures Data
P8-0054 Fig 1

Documentation

Protocol
P8-0054 Protocol LS63
Catalogue NumberDescriptionPack SizePrice
P8-0050FastGene Scriptase II cDNA Synthesis Enzyme100 Reactions£227.5
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