| Catalogue Number | Description | Pack Size | Price |
|---|---|---|---|
| P8-0055 | 5x FastGene Scriptase II cDNA Synthesis Kit Ready-Mix, 100 Rxns | 100 Reactions | £382.00 |
P8-0055
5x FastGene Scriptase II cDNA Synthesis Kit Ready-Mix, 100 Rxns
Engineered reverse transcriptase with a mutation in the RNase H domain and higher thermal stability which is ideal for RT-qPCR and NGS.
Ready-to-use with all necessary ingredients to perform a reverse transcription.
Ready Mix
The FastGene® Scriptase II 5x ReadyMix is an engineered enzyme with a reduced RNase H activity and comes ready-to-use with all necessary ingredients to perform a reverse transcription.Engineered enzyme
Engineered reverse transcriptases allowed the synthesis of cDNA from very low amounts of RNA. Mutations are inserted into the RNase H domain of the MuLV‘s reverse transcriptase. Therefore, by not degrading the RNA during the first-strand synthesis, a higher yield of full-length cDNA is obtained. Additionally, a higher thermal stability increases the robustness of the enzyme. The FastGene® Scriptase II is exactly one of those engineered enzymes. With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS.Lower RNase H activity for longer cDNA
The FastGene® Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12 kBp.Engineered enzymes – optimized for qPCR
The FastGene® Scriptase II delivers superior cDNA templates for downstream applications, e.g. qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants. Engineered reverse transcriptases allowed the synthesis of cDNA from very low amounts of RNA. Mutations are inserted into the RNase H domain of the MuLV's reverse transcriptase. Therefore, by not degrading the RNA during the first-strand synthesis, a higher yield of full-length cDNA is obtained. Additionally, a higher thermal stability increases the robustness of the enzyme. The FastGene® Scriptase II is exactly one of those engineered enzymes. With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS.The FastGene® Scriptase II 5x ReadyMix comes with all necessary ingredients to perform a reverse transcription:
- FastGene® Scriptase II
- 5x FastGene® Scriptase II buffer
- dNTP Mixture
- RNase Inhibitor
- Random Hexamer
- Helper Protein
Expect great results from multi-plex PCR, qPCR, and NGS
The FastGene Scriptase II delivers superior cDNA templates for common downstream applications such as PCR, qPCR, and NGS. The resulting full-length cDNA is of the highest quality and gives a complete picture of the gene and any modification, such as splicing variants. Results using Scriptase II compare very favorably to the most popular brands of reverse transcriptases. For example, complicated multi-plex PCR reactions using cDNA derived from Scriptase II are indistinguishable between brands. In these examples below, GAPDH and YWHAZ were carefully examined via qPCR and both show nearly identical Ct values across a broad range of input RNA concentrations.
Comparison of qPCR results using primers for GAPDH and cDNA produced by using different RNA starting concentration by Competitor I‘s SS-II enzyme and FastGene Scriptase II at 42 °C.
Comparison of qPCR results using primers for YWHAZ and cDNA produced by using different RNA starting concentration by Competitor I‘s SS-II enzyme and FastGene Scriptase II at 42 °C.
Comparison of different PCR results — multiplex PCR
Comparison of multiplex PCR using cDNA produced by Competitor I‘s SS-II enzyme and FastGene Scriptase II at 42 °C and 50 °C.
Specification
| Specification Quantification of Gene Expression. RT-qPCR. Next Generation Sequencing. Low RNA concentration. Difficult templates. The FastGene® Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12 kBp. The FastGene® Scriptase II delivers superior cDNA templates for downstream applications, e.g. qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants. |
Documentation
| Protocol P8-0055 Protocol |
| MSDS P8-0055 MSDS |
| Manual P8-0055 Manual |
