This kit is suitable for the isolation of Total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification Kit – 17270
Specification
Maximum Binding Capacity Per Column
Up to 50 μg RNA
Maximum Loading Volume Per Spin Column
650 μL
Size of RNA Purified
All sizes, including small RNA < 200 nt
Time to Complete 10 Purifications
20 minutes
Maximum Amount of Starting Material
Liver Tissue, 10 mg
Heart Tissue, 5 mg
Kidney Tissue, 10 mg
Brain Tissue, 25 mg
Lung Tissue, 10 mg
Spleen Tissue, 20 mg
Whole Blood, 100 µL
Plasma/Serum, 200 µL
Hela Cells, 3 x 106
CHO , 3 x 106
Yeast, 1 x 108 cells
E.coli, 1 x 109 cells
Bacteria, 1 x 109 cells
Fungi, ≤ 50 mg
Plant Tissues, ≤ 50 mg
Plant Cells, 5 x 106 cells
Viruses, ≤100 µL of viral suspension
Average Yield
HeLa Cells (1 x 106 cells)
E. coli (1 x 109 cells)
15 μg
50 μg
*For isolating Total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (P4-0061-25700)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.
These reagents should remain stable for at least 1 year in their unopened containers.
Figure
Documentation
Literature
| P4-0426 Literature 17270 |
Protocol
| P4-0426 Protocol 17270 |
Manual
Safety Report
MSDS
| P4-0426 MSDS 17270 |
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