Total RNA Purification Kit, 96-Well Kit (Deep Well Format): Scalable High-Throughput RNA Isolation
The Total RNA Purification Kit, 96-Well Kit (Deep Well Format) provides a robust and rapid solution for the high-throughput isolation of total RNA from an extensive range of sample sources. Engineered for efficiency, this system allows researchers to process up to 96 samples in just 30 minutes, making it an essential tool for large-scale genomic studies. The deep-well format is specifically optimised for compatibility with vacuum manifolds, plate centrifuges, and automated liquid handling workstations, ensuring seamless integration into high-capacity laboratory workflows without compromising on yield or purity.
Utilising advanced resin-based technology, the kit employs spin-plate chromatography to bind RNA molecules without the requirement for hazardous phenol or chloroform. This unique mechanism is size-agnostic, allowing for the simultaneous capture of the entire RNA profile—from large mRNA and lncRNA down to microRNA (miRNA) and siRNA—in a single elution fraction. The resin’s high affinity for small RNAs, regardless of their GC content, ensures a truly representative snapshot of the transcriptome, providing high-quality eluates with excellent RIN values for sensitive downstream applications such as NGS, RT-qPCR, and microarrays.
Key Benefits:
- Comprehensive RNA Recovery: Capture the full transcriptome in one fraction. Efficiently isolates all RNA species, including small RNAs (<200 nt) and miRNA, without protocol adjustments.
- High-Throughput Optimisation: Process 96 samples in 30 minutes. The deep-well plate design facilitates rapid processing and higher loading volumes, ideal for automation and vacuum-based systems.
- Phenol-Free Chemistry: Enhance laboratory safety and sustainability. Eliminates the need for toxic organic extractions, reducing hazardous waste and ensuring eluates are free from chemical carryover.
- Superior Purity and Integrity: Optimised for sensitive downstream analysis. Delivers consistent, high-purity RNA with exceptional A260/A280 ratios and RIN values suitable for NGS and qPCR.
- Extreme Versatility: Single-kit solution for diverse inputs. Validated for use with cultured cells, tissues, blood, plasma, serum, bacteria, yeast, and fungi.
Technical Insights
The deep-well architecture of these plates is specifically designed to handle larger sample volumes and prevent cross-well contamination during high-speed centrifugation or vacuum aspiration. This format is particularly beneficial when working with dilute samples or when maximum biomass processing is required to achieve higher total yields. By maintaining consistent well-to-well performance, the kit ensures that experimental variability is minimised across large cohorts.
| Catalogue No. | Product | Contents/Specifications |
|---|---|---|
| P4-0440 | Total RNA Purification Kit, 96-Well Kit (Deep Well Format) | 192 Preps (2 x 96-well plates) |
| P4-0444 | Total RNA Purification Kit, 96-Well Kit (Deep Well Format) | 576 Preps (6 x 96-well plates) |
Specifications
- Maximum Binding Capacity: 50 µg per well
- Maximum Loading Volume: 500 µL per well
- RNA Size Purified: All sizes, including small RNA < 200 nt
- Average Yield (HeLa 1×10⁶ cells): 15 µg
- Time to Complete 96 Purifications: 30 minutes
- Storage Conditions: Room Temperature (stable for 1 year)
- Certification: Consistent well-to-well isolation for NGS/qPCR