ProteoSpin™ On-Column Proteolytic Digestion Kit: Streamlined Sample Prep for Mass Spectrometry
Preparing protein samples for mass spectrometry often involves tedious, multi-step protocols that increase the risk of sample loss and contamination. The ProteoSpin™ On-Column Proteolytic Digestion Kit provides a revolutionary solution by integrating protein purification, digestion, and peptide recovery into a single, streamlined spin-column procedure. This approach eliminates the need for time-consuming overnight dialyses or complex precipitation steps, ensuring that researchers can transition from raw protein samples to MS-ready peptides in a fraction of the traditional time.
The system utilises advanced ion-exchange chromatography within a convenient spin-column format. Proteins are first bound to the column matrix, where contaminants such as salts, detergents, and buffers are washed away. Proteolytic enzymes (such as Trypsin) are then introduced directly onto the column for efficient digestion. Because the digestion occurs in a concentrated environment on the matrix, the reaction kinetics are highly favourable. Following digestion, the resulting peptides are eluted in a small volume of MS-compatible buffer, ready for immediate downstream analysis without further clean-up.
Key Benefits:
- Unified Workflow: Purification and digestion in one. Combines sample clean-up and enzymatic digestion into a single protocol, significantly reducing manual handling time.
- High Peptide Recovery: Maximise your data. The on-column format minimises sample transfer steps, ensuring maximum recovery of even low-abundance peptides for sensitive LC/MS applications.
- Rapid Processing: Results in hours, not days. Accelerated digestion kinetics and efficient elution allow for the completion of the entire process in significantly less time than traditional in-solution methods.
- Superior Purity: Optimised for MS. Effectively removes common MS-interfering substances, including SDS, Triton™ X-100, and high salt concentrations, leading to cleaner spectra and better base calling.
Technical Insights
The ProteoSpin™ technology is engineered to handle a wide range of protein concentrations. By immobilising the protein on the column, the effective concentration of the substrate is increased, allowing for efficient digestion even when using lower ratios of expensive enzymes like Trypsin. The kit is compatible with various proteolytic enzymes, providing flexibility for different proteomic mapping requirements.
Specifications
- Sample Type: Purified proteins or complex lysates
- Format: Spin Column (Ion-exchange chromatography)
- Processing Time: Approximately 3–4 hours (including digestion)
- Downstream Compatibility: LC-MS/MS, MALDI-TOF, HPLC
- Storage Conditions: Room temperature (Enzymes may require -20°C if supplied)