This kit provides everything required to isolate and purify inclusion body Proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilisation Reagent. Inclusion body Proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting.
The procedure is efficient and streamlined and can process up to 12 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic Proteins from 100ml of induced bacterial culture. Purified recombinant Proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit – 17700
Specification
| P4-0024 Specification 17700 |
Figure
Documentation
Literature
| P4-0024 Literature 17700 |
Protocol
| P4-0024 Protocol 17700 |
Manual
Safety Report
MSDS
| P4-0024 MSDS 17700 |
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