Bacterial Genomic DNA Isolation Kit – 17900

Bacterial Genomic DNA Isolation Kit: Universal & Rapid Purification

The Bacterial Genomic DNA Isolation Kit (Catalogue #17900) provides a robust and rapid method for the extraction of high-quality genomic DNA from a broad spectrum of bacterial species. Designed to handle both Gram-negative and difficult-to-lyse Gram-positive bacteria, this kit overcomes common lysis challenges associated with thick peptidoglycan cell walls. By utilising a proprietary spin column chromatography technology, the protocol eliminates the need for hazardous phenol/chloroform extractions or time-consuming alcohol precipitation, delivering purified DNA that is immediately ready for downstream applications.

The system is optimised for research requiring high sensitivity and consistency. It processes up to 2 x 10⁹ viable bacterial cells (approximately 0.5–1.0 mL of broth culture) in under an hour. The isolation process utilises a novel resin that binds DNA under high salt concentrations and releases it under low salt/alkali conditions, ensuring the effective removal of cellular proteinaceous components and inhibitors. The resulting genomic DNA maintains high integrity and purity, making it ideal for sequencing, PCR, qPCR, Southern blotting, and restriction enzyme digestion.

Key Benefits:

• Universal Compatibility: Isolates from all bacterial types. effectively lyses both Gram-negative (e.g., E. coli) and Gram-positive strains (e.g., Bacillus cereus) without requiring separate protocols.
• Rapid Protocol: Results in under 60 minutes. The streamlined spin-column workflow allows for the processing of multiple samples in approximately 30–45 minutes.
• High Yield & Purity: Consistent recovery up to 20 µg. Delivers high-quality DNA (A260/A280 ratio > 1.8) free from RNA and protein contamination.
• Safe Formulation: Eliminates hazardous chemicals. The procedure is entirely free from phenol, chloroform, and other toxic organic solvents, ensuring user safety.

Technical Insights

Figure 1. Isolation of Genomic DNA from both Gram Positive and Gram Negative Bacteria.

The Bacterial Genomic DNA Isolation Kit was used to isolate genomic DNA from 1 mL overnight culture (1 x 10⁹ cells) of the Gram negative bacteria E. coli (Lanes 1 and 2), the lysozyme-resistant Gram positive bacteria B. cereus (Lanes 3 and 4) and the Gram positive bacteria M. luteus (Lanes 5 and 6). Lane M is Norgen’s UltraRanger 1kb DNA Ladder. For analysis 5 µL of the 200 µL eluted genomic DNA were loaded on a 1X TAE, 0.9% agarose gel.

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Figure 2. High Yield Purification.

The high yield of Norgen’s Bacterial Genomic DNA Isolation Kit is illustrated by purifying genomic DNA from 1 mL overnight culture (1 x 10⁹ cells) of both a Gram positive (B. cereus) and a Gram negative strain (E coli), and comparing the yield with two major competitors. The quantification of the DNA yield was performed by resolving 5 µL of the 200 µL of eluted DNA on a 1X TAE, 0.9% agarose gel followed by densitometry. With both types of bacteria, Norgen’s kit was found to give a higher recovery than the competitor’s kits.

Specifications

• Max Input: 2 x 10⁹ bacterial cells (0.5 – 1.0 mL culture)
• Column Binding Capacity: 25 µg
• Average Yield: Up to 20 µg (dependent on species/growth)
• Time to Complete: < 60 minutes (10 purifications)
• Elution Volume: 200 µL
• Storage: Room Temperature (stable for 2 years)