A Gold Standard in DNA Visualisation
Stop compromising between safety and results. Discover the evidence-based replacement for Ethidium Bromide that delivers superior data and dramatically improves your experimental success.
In This Guide, You’ll Learn:
- The comprehensive safety data that makes Midori Green a non-hazardous alternative to Ethidium Bromide.
- How Midori Green’s performance compares directly to EtBr and SYBR Safe in sensitivity and signal-to-noise ratio.
- The critical impact of DNA stain and illuminator choice on downstream applications like cloning.
- How to select the right Midori Green formulation for your specific workflow and instrumentation.
Estimated Read Time: 6 minutes
Pillar 1: Uncompromising Safety
Midori Green’s commitment to safety is validated by extensive third-party testing, ensuring protection for you, your sample, and the environment.
Comprehensive Five-Test Safety Panel
Furthermore, Midori Green is classified as non-hazardous waste, eliminating costly disposal procedures and reducing environmental impact. Read the full third-party safety report here.
Pillar 2: Superior Performance
Achieve publication-quality data with sharper bands, higher sensitivity, and an exceptional signal-to-noise ratio that competing stains cannot match.
Midori Green Advance vs. EtBr (UV Illumination)
Ethidium Bromide
Result: Comparable or greater band intensity than Ethidium Bromide when viewed on a standard UV table, serving as a direct, safer, high-performance replacement.
Midori Green Xtra vs. SYBR Safe (UV Illumination)
SYBR Safe
Result: Under UV, Midori Green Xtra shows a superior signal-to-noise ratio, resulting in clearer bands against a darker background for more accurate analysis.
Midori Green Xtra vs. GelRed (Blue-Green LED)
Gel Red
Result: When paired with the safe Blue-Green LED light of the FAS-X system, Midori Green Xtra‘s performance is maximised, delivering unmatched sensitivity.
Crucially, Midori Green Xtra does not cause the electrophoretic mobility shift seen in other safe stains, ensuring accurate sizing of DNA fragments.
Pillar 3: Enhanced Experimental Outcomes
The biggest benefit is what happens *after* you run the gel. By protecting DNA from damaging UV light, the Midori Green system dramatically improves the success of downstream applications.
Dramatically Increased Cloning Efficiency
This chart shows how Midori Green + Blue/Green LED light protects DNA from damage compared to the traditional EtBr/UV method, leading to a more than two-fold increase in successful cloning colonies.
Pillar 4: Compelling Economic Value
The true value isn’t just the price per gel; it’s the reduction in the “Total Cost of Experimentation” by preventing failed experiments and saving on waste.
Midori’s vs Competitor Value
Midori Green stains are significantly more cost-effective than other major “safe” stains on the market, providing superior performance at a lower price point.
The Complete, Optimised Workflow
Maximise the benefits of Midori Green with a fully integrated and optimised gel documentation system from Geneflow, ensuring perfect compatibility and peak performance.
FastGene® FAS-X
An elegant, stand-alone system for effortless, high-quality imaging with an integrated Blue/Green LED transilluminator.
FastGene® BG LED Transilluminator
The essential partner for sample protection, this safe illuminator perfectly excites Midori Green stains without damaging DNA.
Find Your Perfect Match
Different experiments have different demands. This guide helps you select the ideal Midori Green formulation for your specific application and lab setup.
Midori Green Advance: Optimised for UV Illumination
Best for: Labs wanting to immediately improve safety using their existing UV transilluminators.
A direct, safer replacement for Ethidium Bromide that you can adopt today without changing your hardware. It’s the simplest first step away from hazardous materials. Ideal for routine checks, PCR product verification, and teaching labs.
Midori Green Xtra: Maximum Signal with Blue/Green LED Systems
Best for: Labs with Blue/Green LED systems demanding the absolute highest sensitivity and data accuracy.
Delivers the cleanest background and sharpest bands without causing the electrophoretic mobility shift seen in competing stains, ensuring you can trust your sizing. Essential for applications requiring precise band sizing, such as genotyping, RFLP, and analysis of low-concentration DNA libraries.
Midori Green Direct: The In-Sample Stain for Accelerated Workflows
Best for: High-throughput screening and protocols involving gel extraction for cloning, ligation, or sequencing.
Simply add the stain to your sample, not the gel, for the fastest workflow. Its formulation is optimised to maximise downstream success. The go-to choice for researchers performing molecular cloning, site-directed mutagenesis, and preparing samples for NGS.