1Kb PLUS DNA Ladder, 100bp-10Kb, 500uL: Sharp, Ready-to-Use Sizing
Accurate determination of DNA fragment size is a fundamental requirement for any molecular biology laboratory performing PCR, restriction digests, or molecular cloning. The 1Kb PLUS DNA Ladder provides a comprehensive, highly reliable solution for precisely sizing double-stranded DNA across a broad molecular weight range. Supplied in a generous 500µL volume, this pre-mixed, ready-to-use (RTU) format completely eliminates the need for time-consuming preparation steps such as thawing, diluting, and adding tracking dyes, thereby streamlining your electrophoresis workflow and minimising the potential for pipetting errors.
Formulated to produce exceptionally sharp, well-defined bands, this DNA marker is composed of discrete fragments meticulously isolated from restriction-digested proprietary plasmids. Spanning an extensive range from 100 base pairs up to 10 kilobases (10Kb), the ladder incorporates high-intensity reference bands that serve as immediate, visual orientation points during gel analysis. This allows researchers to effortlessly perform size comparisons for a wide array of samples, from the smallest PCR amplicons to large, linearised cosmid vectors, ensuring absolute confidence in your downstream applications.
Key Benefits:
- Ready-to-Use Convenience: Load directly onto your gel. Pre-mixed with loading dye, enabling immediate application without the need for prior dilution or dye addition, saving valuable preparation time.
- Broad Size Range: Comprehensive 100bp to 10Kb coverage. A highly versatile marker suitable for sizing a wide spectrum of DNA molecules, replacing the need to purchase multiple separate ladders.
- Clear Visual Orientation: High-intensity reference bands. Specially formulated to include prominent reference fragments, allowing for rapid and unambiguous size determination at a glance.
- Exceptional Stability: Flexible storage options. This uniquely stable formulation remains viable for up to 6 months at room temperature, 12 months at 4°C, or 24 months when stored at -20°C, ensuring long-term reliability.
Technical Insights
DNA Sizing and Separation
The 1Kb PLUS DNA Ladder provides optimal resolution when run on standard agarose gels. The discrete fragments resolve cleanly, allowing for the accurate interpolation of sample sizes based on their relative migration distance compared to the known marker bands. Its proprietary plasmid source ensures batch-to-batch consistency for reliable, reproducible analytical results.
Specifications
- Product Type: Ready-to-Use (RTU) DNA Marker
- Size Range: 100 bp to 10,000 bp (10 Kb)
- Volume: 500 µL
- Format: Pre-mixed with loading dye
- Source: Restriction-digested proprietary plasmids
- Room Temperature Stability: Up to 6 months
- Refrigerated Stability (4°C): Up to 12 months
- Freezer Stability (-20°C): Up to 24 months
- Application: Sizing of PCR fragments, restriction digests, and linearised vectors