TAE Buffer (50 X): Optimised for Large DNA Fragment Separation
TAE Buffer (50 X) is the preferred choice for researchers performing agarose gel electrophoresis of high-molecular-weight DNA fragments. While other buffers may offer higher capacity, TAE provides the specific mobility characteristics required to resolve large DNA sequences (>12 kb) with superior clarity. This 50 X concentrate from National Diagnostics offers a convenient, high-purity solution for everyday genomic analysis and DNA purification.
The mechanism of TAE (Tris-Acetate-EDTA) involves a lower ionic strength than TBE, which results in a slower migration of DNA but significantly improved resolution of larger molecules. It is also the ideal choice for applications where the DNA must be recovered from the gel, as the acetate ions do not interfere with downstream enzymatic reactions such as ligation or transformation as much as borate ions do.
Key Benefits:
- Large Fragment Clarity: Superior resolution. Optimised for the separation of large genomic DNA and complex plasmid vectors.
- Downstream Compatibility: Enzyme friendly. The absence of borate makes this buffer ideal for DNA extraction and subsequent cloning procedures.
- Economical 50 X Concentrate: High value. Highly concentrated format reduces shipping costs and laboratory storage requirements.
- Instant Dissolution: No precipitate. Expertly formulated to ensure no salts drop out of solution, even at 50 X concentration.
Specifications
- Concentration: 50 X Liquid Concentrate
- Composition: 2M Tris-Acetate, 0.05M EDTA (pH ~8.3 at 1X)
- Storage: Store at room temperature (15-30°C)
- Shelf Life: 24 months
- Application: Agarose gel electrophoresis, DNA recovery/cloning