50bp DNA Ladder: Precision Resolution for Small Fragment Analysis
The 50bp DNA Ladder is a high-quality molecular weight marker specifically engineered for the precise sizing and quantification of small double-stranded DNA fragments. In molecular biology workflows, distinguishing between closely sized PCR products or restriction fragments can be challenging; this ladder provides a reliable reference scale to ensure data integrity during gel electrophoresis. It is designed to eliminate ambiguity, providing researchers with the confidence needed for routine screening and complex analytical applications.
This ladder consists of seven discrete chromatography-purified DNA bands ranging from 50 bp to 500 bp. To facilitate effortless orientation and rapid identification under UV light, the 250 bp band is increased in intensity, serving as a distinct reference point. The formulation is supplied in a “ready-to-use” format, pre-mixed with loading dye and stable reagents, which eliminates the need for manual dilution or the addition of tracking dyes, thereby reducing the risk of pipetting errors and contamination.
Key Benefits:
- Enhanced Visual Reference: High-intensity 250 bp band. Features a reinforced internal fragment to allow for immediate orientation and easy identification of sizes during the run.
- Streamlined Workflow: Ready-to-load convenience. Pre-mixed with loading buffer and tracking dyes, allowing for direct application from the tube to the gel.
- Broad Compatibility: Optimised for various gel types. Formulated to provide sharp, crisp resolution on both agarose and non-denaturing polyacrylamide gels.
- Quantitative Accuracy: Precise mass determination. Each band contains a known concentration of DNA, enabling the estimation of sample mass based on relative fluorescence.
Technical Insights
Migration Pattern and Band Distribution
The 50bp DNA Ladder provides a linear distribution of fragments (50, 100, 150, 200, 250, 300, and 500 bp). This specific spacing is ideal for monitoring the migration of small PCR amplicons and ensuring high-resolution separation in higher percentage gels (2.0% to 3.0% agarose).
Specifications
- Fragment Range: 50 bp to 500 bp
- Number of Bands: 7
- Reference Band: 250 bp (increased intensity)
- Format: Ready-to-use (includes loading dye)
- Total Loads: 100 (based on 5 µl per well)
- Storage Conditions: Store at 4°C for short-term use; -20°C recommended for long-term stability
- Shelf Life: 12 months from date of receipt when stored correctly