Midori Green Advance: The Professional Standard for Safe Nucleic Acid Staining
Midori Green Advance is a high-performance, non-carcinogenic nucleic acid stain developed as a safer, more efficient alternative to traditional ethidium bromide (EtBr). Designed for the modern molecular biology laboratory, it provides an expert-grade solution for the detection of dsDNA, ssDNA, and RNA in agarose gels, eliminating the health risks and environmental hazards associated with mutagenic dyes. By integrating Midori Green Advance into your workflow, you ensure a safer working environment without sacrificing the sensitivity required for critical research applications.
The stain utilises a highly sensitive fluorescent dye that has been chemically optimised for maximum signal intensity when bound to nucleic acids. It features a broad excitation spectrum, making it perfectly compatible with both traditional UV transilluminators and modern, safe Blue/Green LED illumination systems. Unlike other safe stains that may require specialised equipment, Midori Green Advance delivers high-contrast, green-fluorescing bands with exceptionally low background, even in challenging pre-cast or post-staining protocols.
Key Benefits:
- Independently Verified Safety: A non-carcinogenic and non-mutagenic alternative. Rigorous independent testing, including the Ames test and chromosomal aberration tests, has confirmed that Midori Green Advance is safe for handling and disposal as non-hazardous waste.
- Superior Sensitivity: Detect even the smallest DNA fragments. Providing sensitivity comparable to ethidium bromide, this stain allows for the detection of as little as 0.2 – 0.5 ng of DNA per band, ensuring sharp, clear results for analytical electrophoresis.
- Optimised for UV and LED: Versatile compatibility with existing lab hardware. While perfectly suited for traditional UV light sources, it is specifically designed to achieve unbeatable results with Blue/Green LED technology, preventing DNA damage and improving downstream cloning efficiency.
- Outstanding Value: Exceptional concentration for high-throughput cost-efficiency. With an ultra-high 25,000x concentration, a single 1 mL vial is sufficient to stain up to 25 litres of agarose gel, significantly reducing both purchase and shipping costs over time.
- Flexible Application: Seamless integration into any workflow. Midori Green Advance can be used for both pre-cast (in-gel) staining and post-staining procedures, offering researchers the flexibility to choose the method that provides the highest resolution for their specific samples.
Supporting Data
Figure 1: Sensitivity Comparison
Comparison of DNA band intensity between Midori Green Advance (left) and Ethidium Bromide (right) using a UV-transilluminator. Midori Green Advance provides comparable or superior signal intensity while maintaining a lower background for improved clarity.
Midori Green Advance: Frequently Asked Questions
1. Is Midori Green Advance safer than Ethidium Bromide (EtBr)? Yes, significantly. Independent laboratory tests (Ames test) have confirmed that Midori Green Advance is non-mutagenic and non-toxic. This makes it safer for lab personnel and allows for non-hazardous waste disposal.
2. How do I use Midori Green Advance with my gels? You have two simple options: Pre-casting (add 4-6 µL per 100 mL of molten agarose) for the quickest method, or Post-staining (incubate in TAE/TBE buffer for 10-30 minutes) for the sharpest bands.
3. What type of illuminator should I use for visualisation? Midori Green Advance is highly versatile and provides optimal visualisation with both Blue-Green LED (470-520 nm) and traditional UV transilluminators. Blue-Green LED is recommended to protect DNA sample integrity.
4. Can I use DNA extracted from a Midori Green-stained gel for downstream applications? Absolutely. When visualised with a Blue-Green LED illuminator, the DNA is not damaged by high-energy light, making it perfectly suitable for cloning, ligation, and sequencing.
5. Can I use Midori Green Advance to stain RNA gels? Yes, it can be used to visualise RNA in denaturing agarose gels, although the fluorescence signal may be slightly weaker compared to double-stranded DNA.
Specifications
- Concentration: 25,000x (Highly concentrated solution).
- Excitation Peaks: ~270 nm, ~290 nm, and ~490 nm.
- Emission Peak: ~530 nm.
- Staining Capacity: 1 mL vial stains up to 25 litres of agarose; 50 µL vial stains 1.25 litres.
- Storage: Store at 4°C, protected from light. Stability maintained for at least 2 years; do not freeze.
- Safety Certifications: Ames test negative; Non-carcinogenic; Non-toxic.
- Compatibility: Traditional UV light, Blue LED, and Blue/Green LED transilluminators.
- Note: Not recommended for use with SDS-containing loading buffers due to potential band artefacts.