UreaGel Sequencing Kit: The Complete System for High-Resolution Denaturing PAGE
The UreaGel Sequencing Kit (EC-833) is a comprehensive solution providing all the essential reagents required for the preparation of high-resolution denaturing polyacrylamide gels. Designed for the demanding requirements of DNA sequencing and oligonucleotide analysis, this kit eliminates the complexity of sourcing and mixing individual components. By integrating high-purity acrylamide, urea, and TBE buffer into a modular system, the UreaGel Kit ensures that researchers can achieve unparalleled reproducibility and sharpness in their sequencing ladders while significantly reducing the time spent on reagent preparation.
This kit consists of three perfectly matched components: UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. The unique modular design allows for the precise adjustment of the final acrylamide percentage (from 4% to 20%) while maintaining a constant, optimal concentration of 7.5M urea and 1X TBE throughout the gel matrix. This consistency is vital for preventing the formation of secondary structures in nucleic acids, which can lead to band compression or “smiling” gels. Every component is deionised, filtered, and certified nuclease-free, providing a safe and reliable environment for even the most sensitive genetic samples.
Key Benefits:
- Complete Modular System: All-in-one convenience. Includes the concentrate, diluent, and buffer required to cast multiple denaturing gels without further reagent sourcing.
- Consistent Denaturing Strength: Stabilised 7.5M Urea. Ensures that every part of the kit maintains identical urea levels, providing a uniform environment for high-resolution separation.
- Unrivalled Precision: Sharp, reproducible bands. The high-purity, deionised formulations result in a crystal-clear gel matrix with an exceptionally low signal-to-noise ratio.
- Safety-First Formulation: Zero-dust liquid reagents. Minimises laboratory exposure to neurotoxic acrylamide dust, offering a safer alternative to manual powder weighing.
- Certified Nuclease-Free: Sample protection. Rigorously tested for the absence of DNase and RNase to prevent sample degradation during long-run electrophoresis.
Technical Insights
Kit Configuration and Usage
The EC-833 Kit is a specific combination of National Diagnostics’ premium reagents, designed to provide enough material for a significant number of high-resolution sequencing gels. By following the modular mixing protocol, users can tailor the gel percentage to their specific fragment sizes. For example, a standard 6% sequencing gel is prepared by mixing the components in a specific volumetric ratio, ensuring that the urea and TBE concentrations remain perfectly balanced at every step.
Specifications
- Catalogue Number: EC-833
- Kit Contents: UreaGel Concentrate (19:1 or 29:1), UreaGel Diluent, and UreaGel Buffer
- System Denaturant: 7.5M Urea (constant across all components)
- Buffering Capacity: 10X TBE (Tris-Borate-EDTA) in Buffer component
- Purity: Deionised and 0.2 micron filtered
- Certification: Certified Nuclease-Free (RNase/DNase)
- Storage: Room Temperature (18-25°C), protected from light
- Shelf Life: 12 months from date of manufacture