UreaGel 29:1 System: The Complete Modular Solution for Denaturing PAGE
The UreaGel 29:1 System (EC-829) is a comprehensive, three-component kit engineered for the preparation of high-resolution denaturing polyacrylamide gels. Traditional manual gel preparation often requires the tedious and hazardous process of dissolving urea and weighing out acrylamide powder, which can lead to inconsistencies in gel density and buffering. The UreaGel 29:1 System simplifies this workflow by providing pre-mixed, stabilised liquid reagents that guarantee a reproducible 7.5M urea concentration and a precise 29:1 acrylamide to bis-acrylamide ratio for every run.
This modular system comprises UreaGel 29:1 Concentrate, UreaGel Diluent, and UreaGel Buffer. The 29:1 cross-linking ratio is the industry standard for general DNA sequencing and nucleic acid separation, providing the ideal pore size for clear resolution of a wide range of fragment lengths. By adjusting the volumes of Concentrate and Diluent, researchers can easily customise the final gel percentage from 4% to 20% without the risk of altering the denaturing environment or the TBE buffer strength. Every component is rigorously deionised and 0.2-micron filtered to eliminate ionic impurities and particulates, ensuring an exceptionally low background for high-sensitivity imaging.
Key Benefits:
- Unmatched Reproducibility: Standardised 29:1 Ratio. Provides consistent cross-linking for reliable nucleic acid migration and band sharpess across multiple experiments.
- Pre-Optimised Denaturation: Stabilised 7.5M Urea. The urea concentration is held constant across all three components, preventing “smiling” bands and ensuring complete denaturation of DNA and RNA.
- Superior Lab Safety: Ready-to-use liquid format. Eliminates the risk of inhaling neurotoxic acrylamide dust associated with traditional powder-based gel casting.
- Certified Nuclease-Free: Maximum sample protection. Strictly tested for the absence of DNase and RNase to ensure the integrity of precious genetic samples during long electrophoresis runs.
- Streamlined Protocol: Rapid customisation. The modular nature of the system allows for the fast preparation of any gel concentration, significantly reducing prep time in high-throughput labs.
Technical Insights
The UreaGel Modular Advantage
The UreaGel 29:1 System operates on a simple volumetric mixing principle. To create a gel of a specific percentage, the user refers to the provided mixing chart to combine the Concentrate (Monomer), Diluent (7.5M Urea), and Buffer (10X TBE with 7.5M Urea). This ensures that while the acrylamide percentage changes, the electrochemical environment (1X TBE and 7.5M Urea) remains perfectly consistent, resulting in high-resolution, publication-quality gels.
Specifications
- Catalogue Number: EC-829
- Kit Components: UreaGel 29:1 Concentrate, UreaGel Diluent, and UreaGel Buffer
- Cross-linking Ratio: 29:1 (Acrylamide:Bis-acrylamide)
- System Denaturant: 7.5M Urea (constant across all components)
- Buffering Strength: 10X TBE (Tris-Borate-EDTA) in Buffer component
- Certification: Certified Nuclease-Free (RNase/DNase)
- Storage: Room Temperature (18-25°C), protected from light
- Shelf Life: 12 months from date of manufacture