WESTAR® Hypernova: The Industry’s Brightest HRP Detection Substrate
WESTAR® Hypernova represents the pinnacle of chemiluminescent technology, engineered as the brightest Horseradish Peroxidase (HRP) substrate currently available on the market. Designed specifically for the detection of trace amounts of protein, it offers researchers an unprecedented level of sensitivity that extends into the low-attogram range. By providing an extraordinary light output and an exceptionally high signal-to-noise ratio, Hypernova enables the clear visualisation of faint bands that remain undetectable with standard or even high-sensitivity ECL reagents, making it the definitive choice for the most demanding Western blotting applications.
The system utilises a proprietary double-enhancer formulation that intensifies the HRP-catalysed oxidation of luminol. This advanced chemical mechanism not only boosts the initial signal intensity but also ensures a stable light output with a duration of up to five hours. This extended signal window provides significant flexibility during the imaging process, whether utilising high-resolution CCD imaging systems or traditional X-ray film. Furthermore, the extreme brightness of the substrate allows for a substantial reduction in both sample loading and antibody consumption, preserving precious experimental materials without compromising data quality.
Key Benefits:
- Unrivalled Sensitivity: Detect proteins at attogram levels. Engineered to identify trace targets, Hypernova provides the highest sensitivity in the WESTAR® range, outperforming industry standards for low-abundance protein detection.
- Superior Signal-to-Noise Ratio: Achieve crystal-clear results. The specialised formulation produces a minimal electronic background, ensuring that even the faintest signals are distinct and easily quantifiable.
- Significant Cost Savings: Optimise your primary antibody usage. Due to the extreme light output, researchers can utilise primary antibody dilutions up to 1:200,000 and secondary dilutions up to 1:1,000,000, significantly extending the life of expensive reagents.
- Rapid Image Acquisition: Reduce exposure times from minutes to seconds. The intense signal allows for high-quality image capture in a fraction of the time required by standard substrates, increasing laboratory throughput.
- Quantitative Precision: Wide linear dynamic range. Hypernova delivers a linear response relative to protein concentration (R² > 0.99), ensuring accurate and reproducible quantification across a broad range of protein loads.
Technical Insights
Comparative Signal Intensity and Stability
WESTAR® Hypernova is specifically formulated to provide a signal intensity up to ten times stronger than other top-level ECL substrates. This allows for the detection of the same protein quantity with significantly less exposure time. In comparative studies, Hypernova maintained a measurable and quantifiable signal for over five hours, far exceeding the performance of many competing “femto-level” substrates that suffer from rapid signal decay.
Antibody and Sample Optimisation
Because Hypernova generates such a high signal-to-noise ratio, it allows for the loading of significantly lower amounts of cell lysate. This is particularly beneficial when working with rare samples or primary cells. Additionally, the ability to highly dilute antibodies prevents the “burn-out” effect often seen with high-sensitivity substrates when using standard antibody concentrations, resulting in more consistent and reliable blot results.
Specifications
- Detection Limit: Low attogram to high femtogram
- Signal Duration: 5 hours
- Working Solution Stability: 24 hours at room temperature
- Shelf Life: 1 year at room temperature (Stable for shipping at ambient temperature)
- Primary Antibody Dilution: 1:10,000 – 1:200,000
- Secondary Antibody Dilution: 1:300,000 – 1:1,000,000
- Compatibility: Optimised for CCD imagers; compatible with X-ray film
- Pack Sizes: 2 x 10 mL (200 cm² membrane) or 2 x 50 mL (1000 cm² membrane)