WESTAR Sun: Cost-Effective ECL Substrate for Routine Western Blotting
WESTAR Sun is a high-performance, entry-level enhanced chemiluminescence (ECL) substrate designed for the routine detection of proteins in Western blotting applications. Optimised for mid-picogram sensitivity, it provides a reliable and accessible solution for laboratories requiring consistent results without the high costs associated with premium substrates. WESTAR Sun is particularly suited for high-abundance targets and routine screening where high signal-to-noise ratios and low background are paramount for clear data interpretation.
The technology behind WESTAR Sun utilizes a refined luminol-peroxide system enhanced by Cyanagen’s proprietary dual-enhancer technology. This combination stabilises the peroxidase-catalysed oxidation of luminol, converting the typical rapid “flash” of light into a sustained “glow” signal. This modification not only increases the absolute signal intensity but also extends the light emission duration, providing a wide imaging window that is compatible with both digital CCD imagers and traditional X-ray film. The resulting low background ensures that even at its entry-level positioning, the substrate delivers sharp, quantifiable bands.
Key Benefits:
- Reliable Mid-Picogram Sensitivity: Optimised for Routine Use. Specifically designed for the detection of standard protein concentrations with high clarity.
- Long Signal Duration: Extended Imaging Window. Maintains a stable light output for several hours, allowing for multiple exposures and protocol flexibility.
- Excellent Stability: Five-Day Working Solution. Once mixed, the working solution remains stable for at least five days, minimizing reagent waste in high-throughput environments.
- High Signal-to-Noise Ratio: Superior Contrast. Formulated to yield minimal background interference, ensuring that protein bands remain distinct and easy to quantify.
- Cost-Effective Performance: Laboratory Efficiency. Offers an exceptional performance-to-price ratio, making it the ideal choice for everyday experimental needs.
Technical Insights
Performance Comparison: Protein Sensitivity
Specifications
- Detection Level: Mid-picogram
- Signal Duration: Long (several hours)
- Working Solution Stability: 5 days at 2-8°C
- Primary Antibody Dilution: 1:100 – 1:5,000
- Secondary Antibody Dilution: 1:1,000 – 1:15,000
- Storage: Room Temperature (RT)
- Compatibility: Nitrocellulose and PVDF membranes; CCD imagers and X-ray film