FastGene Western ECL Kit: High-Sensitivity Chemiluminescent Detection
The FastGene Western ECL Kit is a premium enhanced chemiluminescent (ECL) substrate designed for the highly sensitive detection of proteins in Western blotting applications. Specifically formulated for use with Horseradish Peroxidase (HRP)-conjugated secondary antibodies, this kit enables researchers to identify antigens at high femtogram to low picogram levels. It provides an expert solution for laboratories requiring clear, high-contrast results for both abundant and low-expression protein targets without the need for complex protocol optimisation.
Utilising an advanced luminol-based technology, the FastGene Western ECL Kit generates a sustained and intense chemiluminescent signal. Unlike standard substrates that suffer from rapid signal decay, this kit is engineered for an extended emission duration. This stability allows for flexible imaging workflows, ensuring that high-quality data can be captured using either modern digital CCD imaging systems or traditional X-ray film without any perceptible loss in signal intensity or increased background noise.
Key Benefits:
- Superior Sensitivity: Detect femtogram levels. Engineered to identify antigens at extremely low concentrations, ensuring that even faint protein bands are clearly visible and quantifiable.
- Extended Signal Duration: Flexible imaging window. The long-lasting chemiluminescent glow allows for multiple exposures and provides ample time for both digital and film-based documentation.
- Seamless Integration: No optimisation required. The kit is designed for an easy switch from other commercial brands, allowing researchers to upgrade their results without changing their established protocols.
- Versatile Membrane Support: Optimised for PVDF and Nitrocellulose. Provides consistent, low-background performance across all standard Western blotting membrane types.
- Imaging Compatibility: CCD and Film ready. Perfectly balanced for the dynamic range of CCD cameras while maintaining the intensity required for X-ray film detection.
Technical Insights
High-Fidelity Signal Stability
The FastGene Western ECL Kit is balanced to provide a high signal-to-noise ratio, ensuring that secondary antibody detection is specific and free from non-specific background interference. This makes it particularly effective for quantitative Western blotting, where linear signal response and reproducibility are essential for accurate data interpretation.
Specifications
- Catalogue No: K1-0164
- Product: FastGene Western ECL Kit
- Detection Level: High femtogram to low picogram
- Substrate Type: Luminol-based ECL
- Volume: 100ml (Supplied as 2 x 50ml bottles)
- Storage: 4°C for long-term stability
- Signal Duration: Extended (Multiple hours)