BLUeye Pre-Stained Protein Ladder: High-Resolution Molecular Weight Estimation
The BLUeye Pre-Stained Protein Ladder is a sophisticated three-colour protein standard designed for the precise monitoring of protein separation during SDS-polyacrylamide gel electrophoresis. Estimating the molecular weight of proteins and verifying Western transfer efficiency onto membranes (PVDF, nylon, or nitrocellulose) is a critical step in protein analysis. This ladder removes the guesswork by providing a clear, sharp, and consistent reference point across a broad range of applications.
This ladder is composed of 12 pre-stained proteins spanning a wide molecular weight range from 10 to 245 kDa. The proteins are covalently coupled with different chromophores for easy identification; the ladder features one green band at 25 kDa and one red band at 75 kDa, while the remaining ten bands are stained blue. This multi-colour system serves as a visual “anchor,” allowing researchers to easily orient their gels and identify specific protein sizes at a glance during the electrophoresis run.
Key Benefits:
- Broad Range Coverage: Consistent monitoring from 10 to 245 kDa. Provides twelve distinct bands to ensure accurate size estimation for both small and large proteins within a single lane.
- Tri-Colour Identification: Instant orientation and reference. Features high-contrast red (75 kDa) and green (25 kDa) reference bands to prevent lane orientation errors and simplify size tracking.
- Ready-to-Use Convenience: No heating or reduction required. Supplied in a loading buffer for direct application to gels, saving time and reducing the risk of contamination in the lab.
- Optimised for Western Blotting: Validated transfer efficiency. Designed to transfer effectively to common membranes, providing a clear visual confirmation that your protein transfer has been successful.
- High Stability: Long-term performance. Formulated to maintain band sharpness and intensity over extended storage periods, ensuring reliable results every time.
Technical Insights
Migration Patterns and Band Identification
The image above demonstrates the migration pattern of the BLUeye Pre-Stained Protein Ladder on a variety of gel types. Note the distinct separation and high intensity of the 70 – 75 kDa red band and the 22 – 25 kDa green band, which act as clear indicators for molecular weight estimation.
Specifications
- Size Range: 10 – 245 kDa
- Number of Bands: 12 Bands (10, 15, 20, 25 (Green), 35, 45, 60, 75 (Red), 100, 140, 180, 245 kDa)
- Volume: 500 µl (Approx. 250 loads)
- Loading Volume: 2-3 µl for Western blots
- Buffer Composition: 20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M Urea, and 15% (v/v) Glycerol
- Storage Conditions: Store at -20°C for up to 24 months; 4°C for up to 3 months
- Certification: Quality controlled for migration consistency and band sharpness