FastGene MOPS Buffer Pouches: High-Resolution SDS-PAGE Separation
FastGene MOPS Buffer Pouches provide a convenient and highly reliable solution for researchers performing denaturing gel electrophoresis. Formulated specifically as a Tris-MOPS-SDS running buffer, these pre-mixed powder sachets eliminate the inaccuracies and time-consuming nature of weighing individual components. When used in conjunction with Bis-Tris systems, such as FastGene PAGE gels, this buffer ensures consistent, reproducible results, particularly when separating medium-to-large proteins where precise band definition is critical.
The technical advantage of the MOPS (3-(N-morpholino)propanesulfonic acid) system lies in its ability to maintain a more neutral pH during electrophoresis compared to traditional Tris-Glycine buffers. This reduced alkalinity significantly diminishes protein hydrolysis and polyacrylamide matrix degradation during the run. The result is exceptionally sharp band resolution and improved protein stability, allowing for high-intensity, fast electrophoresis runs (typically 30 minutes at 200V) without compromising the integrity of sensitive samples.
Key Benefits:
- Exceptional Resolution: Sharper protein bands. The MOPS buffer system minimises protein diffusion and hydrolysis, delivering publication-quality results with superior clarity.
- Rapid Workflows: Accelerated electrophoresis. Engineered to support high-voltage runs, allowing for complete protein separation in approximately 30 minutes.
- Convenience & Precision: Pre-measured sachets. Each pouch contains the exact formulation for 1 litre of 1X buffer, ensuring batch-to-batch consistency and reducing preparation time.
- Extended Stability: Long shelf life. The stable powder format allows for storage at room temperature for up to 24 months without loss of performance.
- Broad Compatibility: Optimised for Bis-Tris gels. Fully compatible with FastGene PAGE gels and other standard Bis-Tris precast and hand-cast gel systems.
Technical Insights
Optimised Separation Performance
The FastGene Tris-MOPS-SDS formulation is specifically designed to provide a wider separation range for medium and large-sized proteins. By providing a stable ionic environment, the buffer facilitates uniform migration speeds, which prevents “smiling” and other common gel artifacts. For laboratories requiring high throughput, the buffer’s ability to operate under constant 200V ensures that multiple gels can be processed quickly without excessive heat generation.
Specifications
- Format: Dry powder sachets
- Volume: 1 Litre per pouch
- Composition: Tris-MOPS-SDS (50mM Tris, 50mM MOPS, 0.1% SDS, 1mM EDTA)
- Shelf Life: 24 months
- Storage: Room Temperature (+15°C to +30°C)