TransIT®-Jurkat Transfection Reagent: Optimised Delivery for T-Cell Lineages
The TransIT®-Jurkat Transfection Reagent is a specialised formulation designed to overcome the inherent challenges of transfecting Jurkat cells and associated lineages. While Jurkat cells (human T-lymphocytes) are a critical tool in immunological research, they are notoriously difficult to transfect with standard reagents. This reagent is specifically optimised to deliver exceptional transfection efficiency for plasmid DNA in these hard-to-transfect cells, as well as other hematopoietic lines such as THP-1 and RAW264.7.
The reagent employs a high-efficiency technology that maintains low cellular toxicity, ensuring that experimental results are not compromised by cell death or stress. It offers a streamlined workflow that is fully compatible with serum-containing media, meaning no medium changes are required post-transfection. Whether performing transient gene expression studies or generating stable cell lines, TransIT-Jurkat provides the reproducibility and simplicity expected from the trusted TransIT series.
Key Benefits:
- Lineage Specificity: Optimised for Jurkat. Specifically developed to achieve high efficiency in Jurkat cells (acute T-cell leukaemia) and other difficult hematopoietic lines like THP-1 and RAW264.7.
- Experimental Integrity: Low Toxicity. The gentle formulation minimises cytotoxicity, preserving cell health and morphology for reliable data.
- Workflow Efficiency: Serum Compatible. Designed for use in complete growth medium; eliminates the need for medium changes to remove transfection complexes.
- Versatile Use: Transient & Stable. Supports both short-term transient expression assays and the generation of stable cell lines.
- Consistent Performance: Reproducible Results. Delivers consistent transfection efficiency, allowing for dependable comparison between experiments.
Technical Insights
Protocol Optimisation
Transfection efficiency can be fine-tuned by adjusting the reagent-to-DNA ratio. The recommended starting point is 3 µl of TransIT-Jurkat Reagent per 1 µg of DNA. Researchers can optimise performance for specific Jurkat subtypes by titrating the reagent from 1–5 µl per 1 µg of DNA. In some cases, doubling both the amount of reagent and plasmid DNA can further increase protein expression.
Specifications
- Product Type: Transfection Reagent
- Storage Conditions: Store at 4°C. Warm to room temperature and vortex gently before use.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Reagent Compatibility: Compatible with serum-containing medium (complete growth medium).
- Antibiotic Compatibility: Transfection complexes can be added to cells grown in medium containing low levels of antibiotics (0.1-1X).
- Guarantee: Guaranteed performance when used as cited.
- Ordering Information:
- E7-0076: 1 x 0.4 ml (MIR 2124)
- E7-0074: 1 x 1.0 ml (MIR 2120)
- E7-0078: 5 x 1.0 ml (MIR 2125)
- E7-0080: 10 x 1.0 ml (MIR 2126)