CHOgro® Expression Medium: Optimised for High-Density Suspension Culture
CHOgro® Expression Medium is a chemically defined, serum-free formulation specifically developed to facilitate high-density growth and large-scale transfection of suspension CHO cells. Unlike traditional media that require lengthy sequential adaptation protocols, CHOgro® allows for rapid acclimation, significantly reducing the time and labour required to prepare cells for protein production. Its optimised composition supports robust cellular health and division, creating the ideal environment for high-titre transient protein expression.
Manufactured under strict cGMP conditions in an ISO-compliant facility, this medium is animal-origin-free and hydrolysate-free, ensuring batch-to-batch consistency and regulatory compliance. It is the core component of the CHOgro® High Yield Expression System, working in synergy with TransIT-PRO® Transfection Reagent to deliver superior yields of therapeutic candidates. Whether you are working with FreeStyle™ CHO-S or other suspension CHO lines, CHOgro® offers a streamlined path to efficient bioprocessing.
Key Benefits:
- High-Density Growth: Maximise biomass. Supports suspension CHO cell densities ranging from $1 times 10^6$ to $1 times 10^7$ cells/ml, providing a robust platform for scalable protein production.
- Rapid Adaptation: Save weeks of effort. Many suspension CHO cells (e.g. FreeStyle™ CHO-S) can be adapted to CHOgro® immediately post-thaw or within 24 hours via centrifugation and resuspension.
- Regulatory Friendly: Consistent and safe. Fully chemically defined, serum-free, and animal-origin-free formulation minimizes contamination risks and regulatory hurdles.
- Versatile Compatibility: Flexible workflow. Designed for use in shake flasks and spinner applications, supporting both R&D and further manufacturing use.
Technical Insights
CHOgro® Expression Medium is engineered for ease of use. Protocol data demonstrates that cells grown in alternate media formulations can often be adapted simply by centrifuging and resuspending in 100% CHOgro® medium. Once adapted, cells typically achieve >95% viability and a doubling time of ≤24 hours, ensuring they are ready for high-efficiency transfection without delay.
Specifications
- Geneflow Number: E7-0210.
- Mirus Part Number: MIR 6200.
- Format: 1 Litre Bottle.
- Storage Conditions: Store at 4°C, protected from light.
- Required Supplements (Per Litre):
- L-Glutamine: 20 ml (200 mM stock).
- Poloxamer 188: 30 ml (10% stock).
- Quality Standard: Sterile-filtered, animal-origin-free, cGMP manufactured.