EZ-PCR Mycoplasma Detection Kit: Reliable Contamination Screening in Hours
Mycoplasma contamination is a pervasive and often invisible threat in cell culture laboratories, affecting up to 35% of continuous cell lines. These parasitic bacteria can drastically alter cellular metabolism, immunological responses, and signal transduction, rendering experimental data unreliable. The EZ-PCR Mycoplasma Detection Kit offers a rapid, highly sensitive solution to identify contamination before it compromises your research.
Unlike traditional culture methods that take weeks, this kit utilises a ready-to-use PCR Reaction Mix to detect over 20 mycoplasma species, including the most common laboratory contaminants (M. fermentans, M. hyorhinis, M. arginini, M. orale). With an integrated internal control to rule out false negatives caused by PCR inhibition, the EZ-PCR kit delivers definitive results in a fraction of the time, allowing you to maintain a clean and trustworthy culture environment.
Key Benefits:
- High Sensitivity & Specificity: The broad-range primer set is designed to amplify the 16S rRNA coding region of mycoplasma with high efficiency, capable of detecting as few as 10 CFU/mL without cross-reacting with eukaryotic DNA.
- Rapid Results: Replace the 28-day turnaround of direct culture methods with a simple PCR protocol that yields definitive answers in under 5 hours.
- Ready-to-Use Convenience: The kit includes a complete, optimised PCR Reaction Mix containing Taq polymerase, dNTPs, and buffer. Simply add your sample and primers—no need to optimise magnesium concentrations or cycling conditions.
- Internal Control for Confidence: A specific internal control DNA template is provided to verify PCR performance, ensuring that a negative result truly means “clean” and not just “inhibited.”
Supporting Data
Figure 1. Easy Interpretation of PCR Results
Diagnosis Guide:
- 270 bp Band (Positive): Indicates the presence of Mycoplasma contamination.
- 357 bp Band (Internal Control): Confirms that the PCR reaction worked correctly.
Note: If a sample is heavily contaminated, the Internal Control band (357 bp) may fade or disappear due to competition for PCR resources, but the presence of the 270 bp band confirms infection.
Specifications
- Target: 16S rRNA gene of Mycoplasma (e.g., M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, etc.)
- Sensitivity: Detects < 10 CFU/mL
- Format: Ready-to-use PCR Master Mix
- Controls: Positive Control DNA and Internal Control DNA included
- Time to Result: < 5 hours
- Storage: -20°C
- Kit Size: 20 Tests