EZ-PCR Mycoplasma Test Kit

A highly-sensitive and specific PCR-based assay designed to detect a broad range of mycoplasma species in cell cultures and other biological materials
K1-0210 Image
Catalogue NumberDescriptionPack SizePrice
K1-0210EZ-PCR Mycoplasma Test Kit20 Assays£224.00
The EZ-PCR™ Mycoplasma Detection Kit is a highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL. The kit contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, a positive control, and an internal control, all for the simple and efficient discovery of mycoplasma contamination. Samples can be prepared in as little as 10 minutes and results can be easily obtained within just a few hours.
  • Easy to use. A complete PCR reaction mix that contains primers, Taq polymerase, and dNTPs is included.
  • Easy to run. Follow a simple protocol with ready-to-use reagents for as little as 10 minutes of preparation.
  • Easy to interpret. Internal and positive controls are included to ensure efficient and accurate PCR interpretation
Routine mycoplasma testing should be carried out minimally every 3 to 6 months, as well as prior to the incorporation of new cultures from outside sources. EZ-PCR™ Mycoplasma kit detects a variety of mycoplasma species: M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum, and Acholeplasma and Spiroplasma species, with high sensitivity and specificity. Furthermore it does not interfere with media components

Avoid False Negatives - Internal Control Included

The EZ-PCR™ Mycoplasma Detection Kit includes an internal amplification control, a plasmid containing a non-mycoplasma-specific DNA sequence to test for false negatives. This internal control is simultaneously amplified in the tube with cell culture samples, and should be used with all PCR samples, including the negative and positive controls. This combination rules out inhibition from biological material, among other assay malfunctions that lead to misinterpretation of results, namely, false negatives.
Figure 1: Gel electrophoresis results obtained following PCR reaction preparation and amplification using the EZ-PCR™ Mycoplasma Detection Kit. Eight total reactions, including six samples, one negative control, and one positive control were tested, each of which contain the internal control to rule out PCR inhibition (i.e. false negatives). As shown, Sample 4 produced a mycoplasma-positive band at 270bp in addition to the internal control band at 357bp, while Samples 1, 2, 3, 5, and 6 produced only internal control bands and are thus negative for mycoplasma. Image courtesy of WiCell Research Institute.



K1-0210 Literature 20-700-20
K1-0210 MSDS 20-700-20
K1-0210 Protocol
Catalogue NumberDescriptionPack SizePrice