CHOgro® Transfection and Titer Enhancement Kit: Maximise Protein Yields
The CHOgro® Transfection and Titer Enhancement Kit (MIR 6225) is a powerful reagent combination designed to significantly boost transient protein production in suspension CHO cells. This kit pairs the high-efficiency TransIT-PRO® Transfection Reagent with the CHOgro® Titer Enhancer, allowing researchers to achieve industry-leading yields of antibodies and other therapeutic proteins. It is the ideal add-on for users who already utilise the CHOgro® Expression Medium or wish to enhance performance in compatible suspension culture systems.
Engineered for a streamlined workflow, the system enables the addition of the Titer Enhancer at the time of transfection (or up to 24 hours post-transfection), followed by a simple temperature shift to 32°C. This process modulates cellular metabolism to prolong viability and increase specific productivity, often outperforming competitor systems such as ExpiCHO™. Fully chemically defined and animal-origin-free, this kit ensures regulatory compliance and reproducibility for scale-up from shake flasks to bioreactors.
Key Benefits:
- Superior Protein Titers: Boost expression. The combination of TransIT-PRO® and CHOgro® Titer Enhancer delivers multi-fold increases in protein yield compared to reagents alone.
- Streamlined Workflow: Save time. Compatible with a “same-day” protocol where transfection complexes and Titer Enhancer are added immediately, eliminating the need for return trips to the incubator.
- Flexible Timing: Convenient schedule. The Titer Enhancer can be added anywhere from 0 to 24 hours post-transfection without compromising performance.
- Animal-Origin-Free: Regulatory friendly. Both the transfection reagent and titer enhancer are free of animal-derived components, making them suitable for biotherapeutic development.
Technical Insights
The CHOgro® Transfection and Titer Enhancement Kit is validated for high-density transfection (approx. $4 times 10^6$ cells/ml). Internal data demonstrates that shifting the culture temperature to 32°C immediately after adding the enhancer significantly increases protein titers by preserving cell viability over a 7–14 day production run.
Specifications
- Geneflow Number: E7-0240.
- Mirus Part Number: MIR 6225.
- Kit Components:
- TransIT-PRO® Transfection Reagent (1 x 1 ml).
- CHOgro® Titer Enhancer (1 x 20 ml).
- Storage Conditions:
- TransIT-PRO®: Store at -20°C.
- CHOgro® Titer Enhancer: Store at 2°C to 10°C, protected from light.
- Guarantee: Guaranteed for 1 year from the date of purchase.