CHOgro® Complex Formation Solution: Optimised for High-Yield Transfection
The CHOgro® Complex Formation Solution is a specialised, chemically defined buffer designed to facilitate the optimal formation of DNA-transfection complexes. Developed as an integral component of the CHOgro® High Yield Expression System, this solution ensures that TransIT-PRO® Transfection Reagent and plasmid DNA interact efficiently in a serum-free environment, overcoming the inhibitory effects often seen with standard culture media.
Strictly formulated to be hydrolysate-free and animal-origin-free, this solution meets the rigorous regulatory standards required for biotherapeutic development. By standardising the complex formation step, it minimises variability and supports high-efficiency gene delivery into suspension CHO cells, contributing to robust and reproducible protein titers.
Key Benefits:
- Chemically Defined Formulation: Regulatory friendly. Fully chemically defined and animal-origin-free, ensuring compliance with strict manufacturing and development standards.
- Optimised Transfection Environment: Maximises efficiency. specifically formulated to support the stable formation of TransIT-PRO®:DNA complexes, preventing precipitation and inhibition commonly caused by serum or antibiotics.
- Streamlined Workflow: Fast and easy. Enables rapid complex formation at room temperature in just 5 minutes, integrating seamlessly into high-throughput protocols.
- Universal System Compatibility: Proven performance. Validated for use within the CHOgro® High Yield Expression System to deliver industry-leading protein yields in suspension CHO derived cells.
Technical Insights
The CHOgro® Complex Formation Solution is critical for achieving the high transfection efficiencies characteristic of the CHOgro® system. It is used to dilute both plasmid DNA and transfection reagent, ensuring they mix uniformly before being added to the cell culture. This step is essential for avoiding the formation of large aggregates that can reduce transfection efficiency.
Specifications
- Volume: 100 ml.
- Storage Conditions: Store at 4°C, protected from light.
- Shelf Life: As indicated on the product label.
- Formulation: Chemically defined, animal-origin-free, hydrolysate-free.
- Geneflow Number: E7-0212.
- Mirus Part Number: MIR 6210.