CHOgro® Expression System: Optimised High-Titre Production
The CHOgro® Expression System (MIR 6260) is a comprehensive, optimised platform designed for transient, high-titre protein production in suspension CHO-derived cells. This complete system integrates the high-density growth capabilities of CHOgro® Expression Medium with the superior transfection performance of TransIT-PRO® Transfection Reagent, streamlining the workflow from cell culture to protein harvest.
Formulated to be chemically defined, hydrolysate-free, and animal-origin-free, the CHOgro® system eliminates the variability often associated with serum-based or undefined media. It is specifically engineered to support robust cell growth and high transfection efficiency, enabling researchers to generate sufficient yields of therapeutic candidates for preclinical studies with speed and reliability.
Key Benefits:
- High Titre Production: Maximise yields. The combination of optimised medium and high-efficiency transfection reagent enables robust protein expression suitable for preclinical development.
- Rapid Adaptation: Save time. Many suspension CHO cells, such as FreeStyle™ CHO-S, adapt easily to CHOgro® Expression Medium, eliminating labour-intensive sequential adaptation protocols.
- Chemically Defined: Regulatory friendly. The entire system is animal-origin-free and hydrolysate-free, ensuring consistency and compliance with rigorous manufacturing standards.
- Complete Solution: All-in-one kit. Includes CHOgro® Expression Medium, TransIT-PRO® Transfection Reagent, CHOgro® Complex Formation Solution, L-Glutamine, and Poloxamer 188.
Technical Insights
The CHOgro® Expression System is designed for flexibility and ease of use. Cells can be transfected at high densities (approx. 2 x 10⁶ cells/ml), and the protocol supports an optional temperature shift to 32°C post-transfection to further increase specific productivity and decrease protein degradation, particularly for secreted antibodies.
Specifications
- Geneflow Number: E7-0220
- Mirus Part Number: MIR 6260.
- Kit Components:
- TransIT-PRO® Transfection Reagent (1 x 1 ml).
- CHOgro® Expression Medium (2 x 1000 ml).
- CHOgro® Complex Formation Solution (1 x 100 ml).
- Poloxamer 188, 10% Solution (1 x 100 ml).
- L-Glutamine, 200 mM Solution (1 x 100 ml).
- Storage Conditions:
- TransIT-PRO®: -20°C.
- CHOgro® Medium & Complex Formation Solution: 4°C (Protected from light).
- L-Glutamine: -10 to -30°C.
- Poloxamer 188: Room temperature.
- Guarantee: TransIT-PRO® is guaranteed for 1 year from purchase; other components as noted on label.