CHOgro® High Yield Expression System: The Ultimate Platform for Protein Production
The CHOgro® High Yield Expression System (MIR 6270) is the advanced evolution of the standard CHOgro® platform, specifically engineered to maximise transient protein titers in suspension CHO-derived cells. This premium system builds upon the robust growth capabilities of the chemically defined CHOgro® Expression Medium by incorporating the CHOgro® Titer Enhancer—a novel additive that modulates cellular processes to significantly boost protein production.
Designed for streamlined bioprocessing, this all-in-one system combines high-efficiency transfection using TransIT-PRO® with a specialised protocol that supports high-density cell growth. By utilising the included Titer Enhancer and an optional temperature shift to 32°C, researchers can achieve multi-fold increases in yield compared to standard protocols, making it the ideal solution for generating therapeutic candidates for preclinical studies.
Key Benefits:
- Maximised Protein Titers: Boost productivity. Includes the CHOgro® Titer Enhancer, which works in synergy with TransIT-PRO® to deliver industry-leading protein yields.
- High-Density Capability: Scalable performance. Supports transfection at high cell densities (approx. 2–4 x 10⁶ cells/ml), allowing for efficient resource utilisation.
- Simplified Adaptation: Seamless transition. Suspension CHO cells, including FreeStyle™ CHO-S and ExpiCHO™, readily adapt to the medium without the need for laborious sequential protocols.
- Regulatory Compliance: Animal-origin-free. All components, including the Titer Enhancer and TransIT-PRO® reagent, are free of animal-derived components and hydrolysates.
Technical Insights
The CHOgro® High Yield Expression System is validated to produce high titers of various proteins, including secreted antibodies. The protocol recommends adding the CHOgro® Titer Enhancer immediately post-transfection and shifting the culture temperature to 32°C to prolong cell viability and increase specific productivity over a 7–14 day harvest period.
Specifications
- Geneflow Number: E7-0238.
- Mirus Part Number: MIR 6270.
- Kit Components:
- TransIT-PRO® Transfection Reagent (1 x 1 ml).
- CHOgro® Titer Enhancer (1 x 20 ml).
- CHOgro® Expression Medium (2 x 1000 ml).
- CHOgro® Complex Formation Solution (1 x 100 ml).
- Poloxamer 188, 10% Solution (1 x 100 ml).
- L-Glutamine, 200 mM Solution (1 x 100 ml).
- Storage Conditions:
- TransIT-PRO®: -20°C.
- CHOgro® Titer Enhancer: 2°C to 10°C (Protected from light).
- CHOgro® Medium & Complex Formation Solution: 4°C (Protected from light).
- L-Glutamine: -10°C to -30°C.
- Guarantee: Reagents guaranteed for 1 year from date of purchase.