Plasma/Serum RNA/DNA Purification Mini Kit: Phenol-Free Sequential Isolation
The Plasma/Serum RNA/DNA Purification Mini Kit provides a fast, reliable, and highly reproducible procedure for the sequential isolation of circulating RNA, exosomal RNA, and cell-free circulating DNA (cfc-DNA) from a single plasma or serum sample. Characterising nucleic acids from low-abundance liquid biopsy samples frequently presents a dual challenge: limited sample volume and the necessity of running multiple individual extractions, which introduces variability and risks cross-contamination. This kit elegantly addresses these constraints, enabling laboratories to maximise data collection from highly valuable or scarce clinical specimens without compromising nucleic acid yield or integrity.
Purification is achieved via advanced spin column chromatography utilising a proprietary resin matrix as the separation medium. This unique technology allows for the simultaneous binding and subsequent sequential elution of all sizes of circulating RNA—including microRNA (miRNA) and small RNAs—exosomal RNA, and all fragments of cfc-DNA. The entire workflow is completely phenol-free and requires no hazardous chloroform extractions or time-consuming protease treatments, ensuring a streamlined, high-throughput-compatible protocol that provides ultra-pure, inhibitor-free eluates perfectly suited for sensitive downstream applications.
Key Benefits:
- Sequential Isolation: Maximise data from a single sample. Extracts circulating RNA, exosomal RNA, and cfc-DNA sequentially from the same plasma or serum sample, ensuring complete genomic profiling from a single aliquot.
- Phenol-Free Workflow: Safer and faster sample preparation. Eliminates the need for hazardous phenol/chloroform extractions or overnight protease digestions, significantly enhancing laboratory safety and reducing hands-on processing time.
- Unbiased RNA Recovery: Comprehensive small RNA capture. Effectively captures all sizes of circulating and exosomal RNA fractions, including microRNA (miRNA) and small regulatory RNAs under 200 nucleotides, without any sequence or size bias.
- Low Input Requirement: Optimised for scarce biopsies. Efficiently processes fresh or frozen plasma or serum sample inputs ranging from as little as 10 µL up to 200 µL, making it ideal for paediatric and rare clinical research applications.
- High Concentration Elution: Flexible, ultra-pure eluates. Delivers purified nucleic acids in flexible elution volumes between 10 µL and 25 µL, removing downstream enzymatic inhibitors and ensuring high concentration for sensitive assays.
Technical Insights
Co-Purification and Sequential Elution Architecture
The Plasma/Serum RNA/DNA Purification Mini Kit utilizes Norgen’s cutting-edge silicon carbide resin technology, which exhibits a superior affinity for nucleic acids compared to traditional silica matrices. By avoiding alcohol-based precipitation methods, the system prevents the selective loss of low-molecular-weight fragments. This ensures that both circulating small RNAs and highly fragmented cell-free DNA are retained quantitatively. The multi-step washing procedure completely removes complex plasma proteins, lipids, and anticoagulants, ensuring that the sequentially eluted RNA and DNA fractions exhibit exceptional purity and exhibit zero carryover between the two fractions.
Specifications
- Catalogue Number: P4-0167
- Product Format: Spin Column (Mini)
- Sample Type: Fresh or frozen plasma or serum
- Input Volume Range: 10 µL to 200 µL per preparation
- Flexible Elution Volume: 10 µL to 25 µL
- Target Analytes: Circulating RNA, exosomal RNA, microRNA (miRNA), and cell-free circulating DNA (cfc-DNA)
- Extraction Chemistry: Phenol-free spin column chromatography with proprietary resin matrix
- Downstream RNA Compatibility: Reverse transcription qPCR, RT-PCR, Next-Generation Sequencing (NGS), Northern blotting, RNase protection, primer extension, and expression arrays
- Downstream DNA Compatibility: PCR, qPCR, Next-Generation Sequencing (NGS), methylation-sensitive PCR, and Southern blot analysis