P4-0230
MicroRNA (cel-miR-39) Spike-In Kit – 59000

Well-accepted microRNA sequence used for normalization in gene expression studies. Best suited for RNA purification from samples with low RNA abundance including liquid biopsies (plasma/serum/urine etc.) and low cell or tissue inputs
P4-0230-Image
Catalogue NumberDescriptionPack SizePrice
P4-0230MicroRNA (cel-miR-39) Spike-In Kit – 59000Kit£97.00
The amount of RNA that can be extracted from different biological or clinical samples varies greatly. For example, while a few micrograms of RNA could be easily purified from tissues and cells in excess amounts (such as from a few milligrams of tissue), many liquid biopsy samples may yield very low amounts of RNA. In fact, samples such as urine or plasma may yield 1 - 100 ng or less RNA per 100 µl of sample. Such a range of RNA quantity is often below the detection limit of most commonly used techniques for measuring RNA including nano-spectrophotometry and fluorescent nucleic acid stains. As a result, without properly determined RNA concentration, it becomes very difficult to normalize the starting quantity of RNA used in gene expression studies. microRNA (cel-miR-39) Spike-In Kit offers a quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays. The amount of cel-miR-39 RNA recovered after RNA extraction is directly correlated with the amount of total RNA recovered. After reverse transcription (such as with microScript Reverse Transcription system) of the sample RNA (with spike-in), the level of cel-miR-39 can be determined by subjecting the cDNA generated to quantitative PCR (qPCR) using fluorescent nucleic acid stains such as SYBR Green. A cel-miR-39 specific primer is included in the kit. The level of expression of any target transcripts in different RNA samples can now be normalized to the cel-miR-39 transcript level using standard method such as ˆ†ˆ†Ct relative quantification. In addition, the cel-miR-39 RNA is compatible to library Preparation methods (including ligation-based protocols) in Next Generation Sequencing (Small RNA-Seq) workflows. The cel-miR-39 RNA could be used for normalization as well as for tracking library construction efficiency.

Specification

Documentation

Protocol
P4-0230 Protocol 59000
Catalogue NumberDescriptionPack SizePrice
P4-0058Total RNA Purification Kit – 1720050 Preps£329
P4-0069Total RNA Purification Kit – 37500100 Preps£620
P4-0062Leukocyte RNA Purification Kit – 2120050 Preps£396
P4-0060Cytoplasmic & Nuclear RNA Purification Kit – 2100050 Preps£445
P4-0071Cytoplasmic & Nuclear RNA Purification Kit – 37400100 Preps£825
P4-0098RNase-Free DNase1 Kit – 2571050 Preps£155
P4-0159RNase- Free DNase 1 Kit – 25720200 Preps£540.00