This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples Prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µl to 1.5ml. Plasma samples Prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR
The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µl to 100 µl. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids.
The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.