Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit: Streamlined Isolation of High-Quality Biomarkers
The Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit provides a rapid, reliable, and convenient method to purify and concentrate high-quality, inhibitor-free cell-free circulating DNA (cfc-DNA) from fresh or frozen plasma or serum. Handling sample volumes ranging from 1 mL to 4 mL, this kit is specifically designed to capture all sizes of cfc-DNA, from large high molecular weight fragments down to 50 base pairs. Circulating DNA serves as a crucial non-invasive biomarker for oncology research, autoimmune disease monitoring, and fetal DNA screening, making robust and efficient extraction vital for downstream diagnostic accuracy.
Purification is achieved through an optimised two-column method based on spin column chromatography, utilising a proprietary resin separation matrix rather than hazardous phenol or chloroform extractions. The first column accommodates the large input volume of bodily fluids, followed by a rapid concentration step on a secondary mini column. This allows researchers to concentrate the cfc-DNA into a highly flexible elution volume of 25 µL to 50 µL, delivering an ultra-pure sample. The resulting DNA is free of common plasma proteins and PCR inhibitors, rendering it fully compatible with sensitive downstream applications, including quantitative PCR (qPCR), methylation-sensitive PCR, Southern blotting, microarrays, and Next-Generation Sequencing (NGS).
Key Benefits:
- Versatile Input Volumes: Process 1 mL to 4 mL of bodily fluids. Accommodates a wide range of fresh or frozen plasma and serum samples to suit varying experimental requirements.
- Flexible Concentration: Elute in just 25 µL to 50 µL. Concentrates cell-free circulating DNA from large input volumes into a highly concentrated format for sensitive downstream analysis.
- Inhibitor-Free Purity: Ready for demanding applications. Purifies DNA completely free of common plasma inhibitors, ensuring flawless performance in qPCR, NGS, and microarrays.
- Comprehensive Recovery: Isolate all sizes of cfc-DNA. Efficiently captures fragmented cell-free circulating DNA, ranging from high molecular weight fragments down to 50 base pairs.
- Rapid Processing: Complete purifications quickly. The streamlined spin-column format eliminates the need for phenol-chloroform extractions, allowing multiple samples to be processed rapidly.
Specifications
- Sample Type: Fresh or frozen plasma and serum
- Input Volume Range: 1 mL to 4 mL
- Elution Volume: 25 µL to 50 µL
- Size of DNA Purified: ≥ 50 bp
- Anti-Coagulant Compatibility: EDTA, Citrate, or Heparin
- Storage Conditions: All components, including the ready-to-use Proteinase K, are stable for up to 2 years at room temperature.