Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit: High-Concentration cfc-DNA Isolation
The Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit is an advanced solution designed for the isolation of cell-free circulating DNA (cfc-DNA) from large volumes of fresh or frozen plasma and serum. In critical clinical and research applications such as liquid biopsies, the naturally low concentration of cfc-DNA requires the processing of significant sample volumes to ensure reliable downstream detection. This kit solves the challenge of sample dilution by allowing researchers to start with large inputs while maintaining exceptional recovery rates.
The technology utilises a specialised two-column system to handle input volumes ranging from 5 mL to 10 mL. The process begins with a large-format column that efficiently captures total DNA from the bodily fluid sample, which is then transferred and concentrated onto a mini column. This dual-stage spin-column chromatography approach facilitates final elution volumes as low as 25 µl, significantly increasing the concentration of target analytes and removing potential PCR inhibitors.
Key Benefits:
- Large Volume Processing: Extract from 5 mL to 10 mL inputs. Specifically optimised for large-volume samples where target cfc-DNA is present at low concentrations, such as oncology and prenatal research.
- Dual Column Concentration: Achieve high-density final eluates. The innovative two-column workflow allows for elution in as little as 25 µl to 50 µl, ensuring maximum sensitivity for low-abundance targets.
- Superior Purity: Remove inhibitors effectively. The resin-based purification effectively eliminates salts, proteins, and other contaminants, providing inhibitor-free DNA for sensitive molecular assays.
- Broad Application Compatibility: Ready for NGS and qPCR. The purified cfc-DNA is fully compatible with Next-Generation Sequencing (NGS), methylation-sensitive PCR, Southern Blot analysis, and microarrays.
Technical Insights
The isolation of circulating DNA is often hindered by the presence of high levels of proteins and lipids in plasma and serum. This Maxi Kit employs a proprietary resin as an ion exchanger within a spin-column format. By utilising a two-step concentration process, the kit overcomes the volume limitations of standard mini columns while retaining the benefit of small elution volumes. This makes it an ideal choice for biomarker discovery and the study of autoimmune diseases, where high-quality genomic material is paramount.
Specifications
- Catalogue Number: P4-0352
- Input Volume: 5 mL – 10 mL
- Sample Type: Fresh or frozen plasma and serum
- Elution Volume: 25 µl – 50 µl
- DNA Size Range: Capable of capturing both small cfc-DNA fragments and larger genomic DNA
- Applications: NGS, qPCR, NIPT, Methylation analysis, Oncology research
- Storage Conditions: Room temperature