- Isolate all sizes of circulating and exosomal RNA, including microRNA
- Versatile plasma/serum input ranging from 1 mL to 6 mL
- No phenol extractions
- No carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL
- Purify superior-quality and superior-quantity RNA in 45 minutes
- Fully automated purification procedure on Hamilton MicroLab Nimbus
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
Plasma/Serum cfc-RNA Advanced Purification Kit
Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit provides a fast, reliable and simple procedure for isolating the highest quality and quantity of all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal DNA contamination. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-RNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-RNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
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Kit Specifications
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Minimum Plasma/Serum Input
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1 mL |
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Maximum Plasma/Serum Input
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6 mL
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Size of cfc-RNA Purified
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All sizes, including miRNA and small RNA (< 200 nt)
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| Elution Volume |
25-50 µL
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| Time to Complete 10 Purifications |
45 minutes
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| Average Yields* | Variable depending on specimen |
*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.