Plasma/Serum RNA Purification Mini Kit: High-Efficiency Circulating RNA Isolation
The Plasma/Serum RNA Purification Mini Kit provides a fast, reliable, and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 50 µL to 200 µL. This system addresses the critical laboratory challenge of recovering high-quality RNA from low-abundance liquid biopsy samples, which is essential for identifying biomarkers in oncology and various disease states. It is optimised to handle both fresh and frozen samples, ensuring flexibility in clinical research and diagnostic development workflows.
Purification is based on spin column chromatography using a proprietary resin separation matrix. Unlike traditional methods, this technology ensures the binding and elution of all RNA sizes—including microRNA (miRNA) and small RNA (<200 nt)—irrespective of GC content or size. The workflow is entirely phenol-free and does not require carrier RNA, delivering high-purity, inhibitor-free RNA that is concentrated into a flexible elution volume of 10 µL to 25 µL for maximum sensitivity in downstream assays.
Key Benefits:
- Comprehensive RNA Recovery: Isolates all sizes of circulating and exosomal RNA. Captures everything from large mRNA to microRNA and small RNA (<200 nt) without size bias.
- Phenol-Free Protocol: Safe and rapid laboratory workflow. Eliminates the need for hazardous phenol/chloroform extractions or protease treatments, completing 10 purifications in under 20 minutes.
- Inhibitor-Free Purity: Optimised for sensitive downstream applications. Purified RNA is fully compatible with NGS, RT-qPCR, Northern blotting, and expression array assays.
- Concentrated Elution: Maximum analyte sensitivity. Allows for elution in volumes as low as 10 µL, facilitating the detection of low-abundance biomarkers.
- Versatile Compatibility: Broad sample and collection support. Validated for use with plasma or serum prepared from blood collected on EDTA, Citrate, or Streck Cell-Free RNA BCT® tubes.
Technical Insights
The proprietary resin matrix allows for the efficient concentration of fragmented circulating RNA from varied input volumes. By eliminating the requirement for carrier RNA, the kit ensures that the resulting sample is pure and ready for high-depth sequencing or sensitive qPCR without interference from exogenous nucleic acids.
Specifications
- Catalogue Number: P4-0176
- Sample Type: Plasma or Serum (Fresh or Frozen)
- Sample Input Volume: 50 µL to 200 µL
- Minimum Elution Volume: 10 µL
- Maximum Elution Volume: 25 µL
- Preparation Time: 15–20 minutes for 10 samples
- Storage Conditions: Room Temperature (15–25°C)
- Shelf Life: 2 years from date of shipment