Plasma/Serum Circulating & Exosomal RNA Purification Kits (Slurry Format): Isolate Low-Abundance RNA with Unmatched Sensitivity
Extracting high-quality circulating RNA and exosomal RNA from cell-free body fluids is notoriously difficult. Researchers often face a compromise: sacrifice yield due to the low abundance of target RNA, or struggle with PCR inhibitors common in plasma and serum. Traditional column-based methods often clog or fail to capture the full spectrum of RNA sizes, while phenol-chloroform extractions are toxic and tedious.
The Geneflow Plasma/Serum Circulating & Exosomal RNA Purification Kits (Slurry Format) are engineered to overcome these hurdles. By utilising a flexible slurry format, this system maximizes the surface area for binding, ensuring the capture of all sizes of circulating RNA, including microRNA (miRNA) and viral RNA, from variable sample volumes. Whether you are analysing tumour-specific markers or viral loads, this kit delivers the concentrated, inhibitor-free RNA required for sensitive downstream applications like RT-qPCR, ddPCR, and Next-Generation Sequencing (NGS).
Key Benefits:
- Superior Sensitivity: Capture the complete RNA spectrum. The slurry format adapts to the sample volume, capturing trace amounts of circulating RNA and exosomal RNA that fixed-volume columns often miss.
- Inhibitor-Free Purity: Generate publication-quality data. Our proprietary buffer system aggressively removes contaminants and inhibitors found in biofluids, ensuring robust amplification in sensitive PCR assays.
- Versatile Scalability: Process any sample volume. From small pilot studies to large-volume screenings, the slurry format allows for variable input volumes without requiring protocol changes or specialised equipment.
- Rapid Workflow: Save valuable bench time. Purify RNA in under 40 minutes with no phenol extraction or alcohol precipitation required.
Available Kit Formats & Scales
To accommodate specific experimental throughputs and sample volume requirements, this chemistry is available in four distinct formats.
- For Routine Analysis (1 mL – 5 mL input): Plasma/Serum Circulating & Exosomal RNA Purification Kit (Slurry Format) – 42800
- For Large Volume Processing (5 mL – 10 mL input): Plasma Serum Circulating & Exosomal RNA Purification Maxi Kit (Slurry Format) – 50900
- For Small Volume/High Concentration (50 µL – 1 mL input): Plasma Serum Circulating & Exosomal RNA Purification Mini Kit (Slurry Format) – 51000
- For High-Throughput Screening: Plasma/Serum Circulation & Exosomal RNA Purification 96-Well Kit (Slurry Format) – 29500
Supporting Data
Figure 1. Isolation and Detection of Circulating RNA from Different Plasma Volumes
The Plasma/Serum Circulating RNA Purification Mini Kit (Slurry Format) was used to isolate circulating RNA from 0.5 mL, 1 mL and 2 mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. RNA isolated from 0.5 mL plasma is represented by the Red line, RNA isolated from 1 mL plasma is represented by the Green line whereas RNA isolated from 2 mL plasma is represented by the Blue line. The black line corresponds to the no template control.
Figure 2. Effective Isolation of Plasma Circulating RNA from Different Volumes
The Plasma/Serum Circulating RNA Purification Mini Kit (Slurry Format) was used to isolate circulating RNA from 0.5 mL, 1 mL and 2 mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. Average Ct values for the amplification of the 5S rRNA isolated from 0.5 mL plasma is represented by the Red bar, Average Ct values for the amplification of the 5S rRNA isolated from 1 mL plasma is represented by the Green bar whereas Average Ct values for the amplification of the 5S rRNA isolated from 2 mL plasma is represented by the blue bar.
Figure 3. Effective and Consistent Detection of Plasma Exosome RNA
Geneflow’s Plasma/Serum Circulating and Exosomal RNA Isolation Kits can effectively isolate RNA from plasma. Plasma Exosome RNA was isolated from 500 µL of human plasma prepared from blood collected on citrate, EDTA or Heparin in triplicates using the Geneflow Plasma/Serum RNA Isolation Kit (blue), a ExoQuick Exosome Precipitation Reagent (green) and Qiagen’s QIAzol extraction followed by a modified Qiagen’s RNeasy Mini Kit cleanup (red). Stem loop RT-qPCR using primers specific to miR-21 and miR-16 as well as the housekeeping 5S rRNA was performed. In brief, three microlitres of the 100 µL isolated RNA was then subjected to a 20 µL reverse transcription using 5S rRNA, miR-21 and miR-16 stem-loop reverse primer or reverse primer. Three microlitres of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21, human miR-16 and the 5S rRNA. The Geneflow Plasma/Serum Exosome RNA Isolation Kit is the only product that showed consistent detection of all tested transcripts with the highest quality regardless of the type of the anti-coagulant used for blood collection.
Specifications
- Sample Type: Plasma, Serum, Cell-Free Body Fluids
- RNA Size Captured: All sizes, including miRNA, siRNA, mRNA, and viral RNA (< 200 nt to > 1000 nt)
- Technology: Slurry/Bead-based extraction (No Phenol/Chloroform)
- Downstream Compatibility: RT-qPCR, NGS, Northern Blotting, Bioanalyzer/TapeStation
- Storage Conditions: Room temperature (Lyophilised components may require specific storage upon reconstitution, refer to manual)
- Usage: Research Use Only