Water RNA/DNA Purification Kit (0.22 µm): High-Sensitivity Microbial Nucleic Acid Extraction
The Water RNA/DNA Purification Kit (0.22 µm) offers a streamlined and efficient method for isolating microorganisms from environmental water samples. Detecting pathogens or monitoring microbial communities in water is often hindered by low biomass and the presence of inhibitory substances. This kit addresses these challenges by capturing organisms on high-efficiency 0.22 µm filters, ensuring that even the smallest bacteria, fungi, and algae are recovered for comprehensive molecular analysis.
The purification mechanism utilises Norgen’s proprietary Silicon Carbide resin technology, which provides superior binding affinity for nucleic acids compared to traditional silica-based methods. After capturing the microorganisms on the provided filter columns, the samples undergo mechanical homogenisation in bead tubes followed by a rapid spin-column purification. This process simultaneously isolates total RNA and genomic DNA in a single elution, removing organic inhibitors and ensuring the samples are ready for demanding downstream applications such as RT-qPCR and Next-Generation Sequencing (NGS).
Key Benefits:
- Simultaneous Isolation: Dual-stream purification. Extract both total RNA and genomic DNA from the same water sample using a single column, saving time and precious sample material.
- Optimised Pore Size: Comprehensive microbial capture. The 0.22 µm filter columns are designed to capture a broad range of microorganisms, including small bacteria that may bypass larger pore filters.
- Complete RNA Recovery: Full transcriptomic insight. Efficiently purifies all sizes of RNA, from large ribosomal and messenger RNA down to microRNA (miRNA) and siRNA, without phenol or chloroform.
- Inhibitor Removal: PCR-ready results. The advanced resin technology ensures the removal of humic acids and other organic inhibitors commonly found in environmental water samples.
- Rapid Processing: High-throughput efficiency. Complete the entire purification for 10 samples in under 45 minutes, significantly accelerating laboratory workflows.
Technical Insights
High Yield and Purity of RNA and DNA
Experimental data demonstrates the kit’s ability to isolate high concentrations of high-quality nucleic acids from E. coli-spiked water samples. Gel electrophoresis confirms the presence of intact genomic DNA alongside clear 16S and 23S ribosomal RNA bands, indicating minimal degradation during the homogenisation and purification steps.
Detection Sensitivity via Real-Time PCR
The purity of the isolated DNA and RNA is validated through real-time PCR (SYBR Green). Even at low microbial concentrations, the absence of inhibitory effects allows for reliable detection of 16S rDNA. The consistency across increasing microbial loads proves the kit’s suitability for sensitive environmental monitoring and pathogen detection.
Specifications
- Catalogue No.: P4-0096
- Prep Size: 25 Preps
- Water Input Range: 10 mL to 100 mL
- Filter Pore Size: 0.22 µm
- Processing Time: 45 minutes for 10 purifications
- Downstream Applications: PCR, qPCR, RT-PCR, qRT-PCR, NGS, Northern/Southern Blotting
- Storage Conditions: Room Temperature (15-25°C)
- Shelf Life: 2 years from date of shipment