Soil DNA Isolation Plus Kit: High-Yield Metagenomic DNA from Challenging Soil Types
The Soil DNA Isolation Plus Kit is a robust solution for the rapid extraction of total genomic DNA from a diverse range of environmental samples. Soil microbiology is frequently hampered by high concentrations of humic acids and other polyphenolic compounds that act as potent PCR inhibitors. This kit is specifically engineered to overcome these challenges, allowing researchers to process even the most difficult samples—such as compost, manure, and clay—ensuring that the resulting DNA is of sufficient purity for sensitive downstream molecular applications.
Purification is achieved through an advanced spin column chromatography system that eliminates the need for hazardous phenol-chloroform extractions. The workflow incorporates a proprietary Organic Substance Removal (OSR) Solution, which selectively precipitates humic acids and organic contaminants during the lysis phase. Following inhibitor removal, DNA is captured on a high-affinity silica-based resin, washed to remove residual salts, and eluted in a low-salt buffer. This mechanism ensures the isolation of high-quality DNA from bacteria, fungi, and algae with minimal shearing.
Key Benefits:
- Universal Soil Compatibility: Optimised for all environmental matrices. Successfully process regular topsoil as well as challenging samples like manure and compost with high organic content.
- Superior Inhibitor Removal: Effective OSR technology. The included Organic Substance Removal Solution eliminates humic acids and polyphenols that typically interfere with PCR and NGS.
- Rapid Workflow: Streamlined sample processing. Complete 10 purifications in just 30 minutes using a convenient spin-column format without the need for specialised equipment.
- High-Quality Yields: Maximum purity for downstream success. Isolated DNA is free from contaminants and fully compatible with PCR, qPCR, and Next-Generation Sequencing.
Technical Insights
The Soil DNA Isolation Plus Kit utilizes a bead-beating lysis method to ensure the efficient release of genetic material from even the most resilient microorganisms, including fungal spores and Gram-positive bacteria. By integrating the OSR step early in the protocol, the system prevents the co-purification of humic substances that often give soil-derived DNA a brown tint and inhibit enzymatic reactions. The resulting purified DNA is eluted in a flexible volume, allowing for the concentration of samples when low microbial loads are expected.
Specifications
- Catalogue Number: P4-0224
- Input Capacity: Up to 250 mg of soil per preparation
- Column Binding Capacity: 50 µg
- Maximum Loading Volume: 650 µL
- Processing Time: Approximately 30 minutes for 10 preps
- Target Microorganisms: Bacteria, Fungi, and Algae
- Storage Conditions: All kit components are stored at room temperature (15-25°C)
- Shelf Life: 1 year from the date of shipment