UreaGel Loading Buffer: Superior Denaturing for PAGE Analysis
UreaGel Loading Buffer is a specialised denaturing solution designed for the preparation of DNA and RNA samples prior to polyacrylamide gel electrophoresis (PAGE). The primary challenge in nucleic acid analysis is the formation of secondary structures or re-annealing of strands, which can lead to multiple bands or smeared results. UreaGel Loading Buffer solves this by ensuring that every sample is fully denatured and remains in a single-stranded state during the loading process.
The formulation contains a high concentration of Urea (8M), a powerful chaotropic agent that disrupts hydrogen bonding between base pairs. In addition to the denaturant, the buffer includes Bromophenol Blue and Xylene Cyanol tracking dyes, which allow for the visual monitoring of the electrophoresis run. The high density of the buffer ensures that samples settle neatly into the wells without cross-contamination or “puffing” into the running buffer.
Key Benefits:
- Complete Denaturation: Maintains single-stranded state. High-concentration 8M urea ensures secondary structures are eliminated for accurate sizing.
- Precision Loading: High-density formulation. Samples sink quickly and evenly to the bottom of the well, preventing sample loss.
- Visual Tracking: Integrated dual-dyes. Contains Bromophenol Blue and Xylene Cyanol to track the migration progress across different fragment sizes.
- Consistency: Batch-to-batch reliability. Pre-mixed and filtered to ensure uniform performance and no interference with sensitive sequencing reactions.
Specifications
- Urea Concentration: 8M
- Dyes: Bromophenol Blue, Xylene Cyanol
- Format: Ready-to-use liquid
- Storage: Store at 4°C for long-term stability; Room temperature for daily use.
- Application: Denaturing PAGE, RNA/DNA sequencing, Oligonucleotide analysis