TransIT®-Oligo Transfection Reagent: Versatile Oligonucleotide Delivery
The TransIT®-Oligo Transfection Reagent is a broad-spectrum formulation designed specifically to enable high-efficiency delivery of oligonucleotides and oligoribonucleotides into a wide variety of cell types. This versatile reagent is capable of transfecting diverse oligonucleotide chemistries, including phosphodiester DNA, phosphothioate DNA (sDNA), phosphothioate RNA (sRNA), 2’OMe RNA, 2’OMe RNA/sDNA chimerics, and morpholino/DNA duplexes. It is an essential tool for researchers conducting antisense studies, gene knockdown, or other applications requiring precise intracellular delivery of short nucleic acid sequences.
Combining the trusted attributes of the TransIT series, this reagent offers high transfection efficiency with exceptionally low toxicity, ensuring that cellular responses are driven by the experimental variable rather than reagent-induced stress. The protocol is designed for simplicity and reproducibility; it does not require medium changes and is fully compatible with serum-containing media. This allows for a streamlined workflow where complexes can be added directly to cells in their complete growth environment, maintaining optimal physiological conditions.
Key Benefits:
- Versatile Compatibility: Broad Spectrum Delivery. Effectively delivers various oligonucleotide types, including sDNA, sRNA, 2’OMe RNA, and morpholinos.
- Superior Cell Health: Low Toxicity. Gentle formulation maintains cell viability and morphology, crucial for interpreting functional assay results.
- Streamlined Workflow: Serum Compatible. Eliminates the need for media changes or serum-free incubation; complexes are added directly to complete growth medium.
- Consistent Performance: High Efficiency. Optimised to provide robust and reproducible delivery across many cell lines.
- Convenient Storage: Ready to Use. Stored at 4°C, avoiding the need for freeze-thaw cycles associated with -20°C reagents.
Technical Insights
Optimisation Guidelines
To ensure optimal performance, the TransIT-Oligo protocol provides specific starting conditions based on the type of oligonucleotide used. For 2’OMe RNA transfection, a starting concentration of 2 µM (final concentration) is recommended, with an optimisation range of 0.5–5 µM. For sDNA oligos, a lower starting concentration of 100 nM is suggested, typically ranging between 50–200 nM. The reagent volume can also be titrated (e.g., 2–5 µl per well in a 24-well plate) to balance efficiency with cell density.
Specifications
- Product Type: Transfection Reagent (Oligonucleotide specific)
- Storage Conditions: Store tightly capped at 4°C. Warm to room temperature and vortex gently before use.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Reagent Compatibility: Compatible with serum-containing medium (complete growth medium).
- Antibiotic Compatibility: Transfection complexes can be added to cells grown in medium containing low levels of antibiotics (0.1–1X).
- Ordering Information:
- E7-0136: 1 x 0.4 ml (MIR 2164)
- E7-0134: 1 x 1.0 ml (MIR 2160)
- E7-0138: 5 x 1.0 ml (MIR 2165)
- E7-0140: 10 x 1.0 ml (MIR 2166)