Label IT® RNAi Delivery Control, Cy®3: Visualise and Optimise Delivery
The Label IT® RNAi Delivery Control (Cy®3) is an essential tool for visualising and optimising the delivery of double-stranded RNA (dsRNA) oligonucleotides. Consisting of Cy3-labeled dsRNA duplexes, this control mimics the physicochemical properties of functional siRNA, allowing researchers to accurately monitor cellular uptake and intracellular distribution. It is particularly useful for verifying effective delivery before committing to expensive or time-consuming functional assays.
The control features a sequence that is not homologous to any known mammalian gene, ensuring that it serves as a true delivery marker without inducing off-target gene silencing. This inert nature makes it suitable for co-transfection alongside experimental siRNA. The Cy3 fluorophore offers bright, stable fluorescence suitable for various imaging applications, from standard fluorescence microscopy to in vivo biodistribution studies.
Key Benefits:
- Direct Visualization: Cy3 Labeled. Provides bright fluorescent tracking of siRNA delivery (Excitation: 550 nm, Emission: 570 nm).
- Optimised Design: siRNA Mimic. Duplexes mimic the length, charge, and configuration of standard siRNA for relevant delivery data.
- Inert Sequence: Non-Targeting. Non-homologous sequence prevents interference with target gene inhibition studies.
- Versatile Application: In Vitro & In Vivo. Ideal for monitoring uptake in cell culture or tracking tissue distribution in animal models.
- High Compatibility: Universal Use. Compatible with a wide variety of transfection reagents and delivery methods.
Technical Insights
Optimisation Guidelines
To achieve the best visualization results, users should titrate the amount of Label IT® RNAi Delivery Control used in their experiment. A recommended starting range for in vitro transfection is 10–100 nM. The fluorescent signal is typically evaluated between 4 and 48 hours post-transfection to ensure accurate assessment of delivery efficiency.
Specifications
- Product Type: RNAi Delivery Control
- Label: Cy3 (Excitation: 550 nm, Emission: 570 nm)
- Format: 100 µg (~7.5 nmol) in RNAi Dilution Buffer
- Concentration: 10 µM stock
- Storage Conditions: Store at -20°C, protected from light.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Ordering Information:
- E7-0194: 1 x 100 µg (MIR 7901)