Label IT® RNAi Delivery Control, Fluorescein: Visualise and Optimise Delivery
The Label IT® RNAi Delivery Control (Fluorescein) is a powerful tool designed to facilitate the visualization and optimisation of double-stranded RNA (dsRNA) oligonucleotide delivery during both in vitro and in vivo RNAi experiments. This control consists of fluorescein-labeled dsRNA duplexes that mimic the length, charge, and configuration of standard siRNA used in research. It allows researchers to directly monitor cellular uptake and intracellular localization, ensuring that their delivery methods are performing effectively before proceeding with functional gene silencing studies.
The sequence of the Label IT® RNAi Delivery Control is engineered to be non-homologous to any known mammalian gene, ensuring it does not interfere with specific target gene inhibition or cause off-target effects. This makes it an ideal co-delivery partner for gene-specific siRNA. Whether used in standard cell culture transfection or advanced in vivo applications such as hydrodynamic tail vein injection, this control provides a reliable fluorescent marker for tracking delivery efficiency.
Key Benefits:
- Direct Visualization: Fluorescein Labeled. Enables easy fluorescent tracking of siRNA delivery in cells and tissues (Excitation: 492 nm, Emission: 518 nm).
- Optimised Design: siRNA Mimic. Double-stranded RNA duplexes possess similar length and charge to standard siRNA, ensuring relevant delivery behavior.
- Inert Sequence: Non-Targeting. Sequence is not homologous to mammalian genes, allowing for co-delivery without affecting target knockdown.
- Versatile Application: In Vitro & In Vivo. Suitable for visualising uptake in cell culture or tracking biodistribution in animal models.
- Flexible Use: Universal Compatibility. Can be delivered using a wide range of transfection reagents or electroporation methods.
Technical Insights
Optimisation Guidelines
To obtain the optimal fluorescent signal, it may be necessary to titrate the amount of Label IT® RNAi Delivery Control. For in vitro experiments, a starting concentration range of 10–100 nM is recommended. The distribution of the fluorescent signal should be assessed between 4 and 48 hours post-transfection to capture the peak delivery window.
Specifications
- Product Type: RNAi Delivery Control
- Label: Fluorescein (Excitation: 492 nm, Emission: 518 nm)
- Format: 100 µg (~7.5 nmol) in RNAi Dilution Buffer
- Concentration: 10 µM stock
- Storage Conditions: Store at -20°C, protected from light.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Ordering Information:
- E7-0196: 1 x 100 µg (MIR 7903)