RNA Clean-Up & Concentration Kit: Maximise Yield and Purity for Sensitive Downstream Applications
The Geneflow RNA Clean-Up & Concentration Kit provides a rapid and reliable solution for the purification and concentration of RNA from various enzymatic reactions. In many molecular workflows, the presence of enzymes, buffers, salts, and unincorporated nucleotides can severely inhibit downstream analysis. This kit is specifically designed to isolate high-quality RNA from post-reaction mixtures, such as DNase I treatment, in vitro transcription, and labelling reactions, ensuring your samples are free from contaminants that could compromise your results.
Utilising advanced silica-membrane spin column technology, the kit facilitates the selective binding of RNA in the presence of chaotropic salts. This mechanism allows for the efficient removal of proteins and chemical impurities while retaining the integrity of the RNA. The unique column design enables elution in very small volumes, allowing researchers to significantly concentrate their samples without the need for time-consuming precipitation steps. The result is pure, concentrated RNA ready for immediate use in the most sensitive applications.
Key Benefits:
- High Recovery: Maximise sample retention. Achieves up to 95% recovery of RNA, ensuring that valuable samples are preserved even when starting with low input amounts.
- Superior Concentration: Optimised for low volumes. The column design allows for elution in as little as 6 µl, yielding highly concentrated RNA essential for microarrays and Next-Generation Sequencing (NGS).
- Rapid Protocol: Streamlined workflow. The simple bind-wash-elute procedure takes only minutes to complete, significantly reducing hands-on time compared to traditional organic extraction methods.
- Effective Decontamination: Ensure reaction success. Completely removes DNase I, proteins, salts, and unincorporated nucleotides, preventing inhibition in downstream applications like RT-PCR and cDNA synthesis.
- Versatile Application: One kit for multiple needs. Suitable for cleaning up RNA after various enzymatic treatments, buffer exchange, and concentrating RNA from dilute solutions.
Technical Insights
Optimised Silica Technology
The purification process relies on a specialised buffer system that optimises the binding conditions for RNA molecules larger than 200 nucleotides. This size selection helps to exclude very small fragments and nucleotides while capturing full-length RNA transcripts. The wash buffers are formulated to aggressively remove salts and organic carryover, ensuring the final eluate has an A260/280 ratio consistently above 1.8.
Workflow Efficiency
Unlike phenol-chloroform extraction, which involves hazardous chemicals and phase separation risks, this spin column format offers a safe and reproducible alternative. The entire process is performed at room temperature, and the absence of carryover ethanol in the final step is guaranteed by an optimised spin-drying procedure.
Specifications
- Format: Mini Spin Column
- Sample Input: Enzymatic reactions, aqueous phase from organic extractions
- Binding Capacity: Up to 35 µg RNA
- Elution Volume: 6 µl – 20 µl
- Processing Time: ~5 minutes
- Recovery Rate: 85% – 95%
- Storage Conditions: Room temperature (15-25°C)