ProtoMetrics Recombinant Protein Markers: High-Precision Molecular Weight Standards
ProtoMetrics Recombinant Protein Markers are an essential tool for the accurate estimation of molecular weight and the monitoring of migration progress during SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Specifically engineered for high-resolution performance, these markers provide a clear and reliable reference frame for researchers performing protein analysis. By using precisely sized recombinant proteins, ProtoMetrics ensures that each band serves as a definitive milestone, eliminating the ambiguity often associated with natural protein mixtures.
The technical foundation of this kit consists of nine recombinant proteins covering a broad range from 10 kDa to 225 kDa. Unlike standard ladders, these recombinant proteins are expressed and purified to ensure consistent mass and high purity, resulting in sharp, distinct bands upon staining. Supplied in a 0.5 ml vial, the marker is optimised for efficiency, providing enough material for 100 mini-gel applications, making it a cost-effective and dependable choice for both routine screening and complex proteomic research.
Key Benefits:
- Broad Range Coverage: Accurate sizing from 10 to 225 kDa. The wide molecular weight range ensures compatibility with a vast array of target proteins, from small peptides to large complexes.
- High Precision: Engineered recombinant proteins. Unlike native protein ladders, these markers provide exact, reproducible band positions for consistent experimental data across different gel runs.
- Ready-to-Use Format: Saves preparation time. Supplied in a convenient quantity of 0.5 ml per vial, the markers are ready for immediate loading onto mini-gels without additional dilution steps.
- Exceptional Clarity: Sharp, distinct banding. The purity of the recombinant proteins ensures that each band remains narrow and well-defined, facilitating precise molecular weight calculation.
- Superior Economy: 100 mini-gel capacity. A single vial provides high-frequency usage, offering excellent value for busy laboratories with high-throughput electrophoresis needs.
Technical Insights
Banding Pattern and Identification
The ProtoMetrics Protein Marker provides a comprehensive reference with nine clearly defined bands. The markers are designed to produce a predictable migration pattern, allowing for the rapid identification of molecular weights during or after staining. The distribution of bands is optimised to provide clear separation, particularly in the mid-range region where the majority of standard research proteins migrate.
Specifications
- Range: 10, 15, 25, 35, 50, 75, 100, 150, and 225 kDa
- Total Proteins: 9 precisely sized recombinant proteins
- Volume: 0.5 ml per vial
- Application Capacity: Sufficient for 100 mini-gels
- Compatibility: Suitable for SDS-PAGE and Western Blotting applications
- Storage: Store at -20°C for long-term stability